甘蓝NAC转录因子BoNAC2的克隆及表达分析  

Cloning and expression analysis of NAC transcription factor gene BoNAC2 in Brassica oleracea L.

作　　者：连蔚然 王晋芳[1] 李殿波[1] 石锦[1] 徐凤凤[1] 曹芸运 赵冰[1] 郭仰东[1] 

机构地区：[1]中国农业大学农学与生物技术学院/设施蔬菜生长发育调控北京市重点实验室,北京100193

出　　处：《中国农业大学学报》2015年第6期124-130,共7页Journal of China Agricultural University

基　　金：国家重点基础研究发展计划(2012CB113900);北京市现代农业产业体系(BLVT-03);北京市自然科学基金(6152015)

摘　　要：为探索甘蓝(Brassica oleracea L.)在各种非生物逆境条件下NAC转录因子的表达,以"中甘11号"为材料,克隆获得甘蓝NAC转录因子BoNAC2基因的cDNA全长序列。序列分析表明,该cDNA片段全长为1 002bp,编码333个氨基酸,具有典型的NAC类蛋白的结构特征。进化树分析表明,该蛋白属于SENU5亚族,并与甘蓝型油菜(Brassica napus L.)BnNAC亲缘关系最近。亚细胞定位显示,BoNAC2蛋白分布于细胞核中。qRT-PCR分析表明,BoNAC2基因受PEG和NaCl诱导表达。NAC are plant-specific transcription factors with multiple biological functions which play important roles in growth and development,stress and hormonal regulation of plant.To study the expression of NAC transcription factor genes under abiotic stress,a full length cDNA of BoNAC2 was isolated from Brassica oleracea of‘zhonggan 11＇.Sequence analysis showed that the full-length of cDNA sequence was 1 002 bp which encoded a 333-amino-acid polypeptide and had the typical characteristics of NAC family.Phylogenetic tree analysis indicated that NAC gene belonged to the SEUN5 subfamily and was closely related to BnNACgene of Brassica napus L.Subcellular localization assay indicated that BoNAC2 was located in nucleus.Quantitative RT-PCR results showed that the expression of BoNAC2 was induced by PEG and NaCl.

关 键 词：甘蓝 NAC转录因子 基因克隆 非生物逆境 荧光定量PCR 

分 类 号：S653.1[农业科学—果树学] Q781[农业科学—园艺学]