分子标记辅助选择抗褐飞虱基因改良桂农占的BPH抗性  

Improving BPH-resistance of Rice Cultivar Guinongzhan by Marker-Assisted Selection for BPH-Resistant Genes

作　　者：胡巍[1] 李艳芳[2] 胡侃 江奕君[1] 张扬[2] 

机构地区：[1]广东省水稻育种新技术重点实验室,广东省农业科学院水稻研究所,广州510460 [2]广东省农业科学院植物保护研究所,广东省植物保护新技术重点实验室,广州510460 [3]广东省种子管理总站,广州510500

出　　处：《分子植物育种》2015年第5期951-960,共10页Molecular Plant Breeding

基　　金：广东省农业科学院院长基金项目(201102);国家863计划项目(2012AA101101);农业部公益性行业(农业)科研专项经费项目(201403002-7);广东省农业科技创新推广与信息化建设专项资金(粤财农[2014]492号)共同资助

摘　　要：本研究通过分子标记辅助选择和连续回交的方法将来源于栽培稻的抗褐飞虱基因Bph3、来源于野生稻的抗褐飞虱基因Bph14和Bph15分别转入到华南高产水稻品种桂农占中,在BC3F3获得了含单个抗性基因的稳定株系和含Bph14和Bph15的稳定株系,其遗传背景与桂农占相似达95%以上。这些株系苗期对褐飞虱的抗性评价表明,在含单个抗性基因的株系中,含Bph3的株系抗性水平最高,含Bph14的最低,而含Bph14和Bph15的株系抗性水平高于含单个基因的株系。对农艺性状的调查发现,含抗性基因的株系与桂农占在抽穗期方面表现出显著差异。在单株产量方面,含抗褐飞虱基因的株系与桂农占没有显著差异。研究结果可为培育抗褐飞虱基因高产水稻品种提供材料。In this study, by marker-assisted selection and backcross breeding, three BPH resistance genes from cultivar rice（Bph3） and wild rice（Bph14 and Bph15） were transferred into indica rice cultivar Guinongzhan, which is susceptible to BPH, to improve its BPH resistance. In BC3F3, lines carrying Bph3, Bph14, Bph15, Bph14 and Bph15, respectively, were obtained. These lines had more than 95% genetic background of Guinongzhan, based on analysis of 188 SSR markers of rice genome. The BPH resistance of these lines was evaluated using seedbox screening, and main agronomic traits of these lines was also evaluated. The results showed that among the lines carrying single gene, the lines carrying Bph3 had the highest resistant level, the line carrying Bph14 had the lowest resistant level. In addition, the lines carrying Bph14 and Bph15 had higher resistant level than any line carrying one gene（Bph3, Bph14 and Bph15）. The lines had significant difference from Guinongzhan in heading date. Compared with Guinongzhan, the lines carrying Bph3 and Bph14 had later heading date, and Bph15 had earlier heading date.The lines carrying BPH resistance gene（s） had no significant difference from Guinongzhan in grain yield. These results are useful for breeding BPH resistant indica rice cultivar with high-yield in South China.

关 键 词：褐飞虱(Nilaparvata LUGENS Stal) 抗褐飞虱基因 分子标记辅助选择 回交 

分 类 号：S511[农业科学—作物学]