水稻苗期耐冷相关性状QTLs的初步定位  

Preliminary Mapping the QTLs for Cold Tolerant-Associated Traits in the Seedling Stage of Rice

作　　者：刘欣[3] 覃宝祥[2] 曾伟[1] 王永亮[1] 王君[1] 江祈贵 陈保善[1] 罗继景[1] 

机构地区：[1]广西大学生命科学与技术学院,亚热带生物资源保护与利用国家重点实验室,南宁530004 [2]广西大学农学院,南宁530004 [3]中国科学院广西植物研究所,桂林541006

出　　处：《分子植物育种》2015年第5期968-976,共9页Molecular Plant Breeding

基　　金：广西自然科学基金(2013GXNSFCA019007);教育部留学回国人员启动基金(教外司留【2013】1792号)共同资助

摘　　要：本研究以粳稻品种＂藤坂5号＂与籼稻品种＂江西丝苗＂为亲本杂交构建的F2分离群体（137个株系）为作图群体,对F2：3家系的3叶期水稻幼苗冷处理（10℃/8℃,昼/夜）5 d、恢复培养7 d后的耐冷级别、叶枯萎度及苗成活率进行完备区间作图。在分子标记连锁分析过程中共检测到6个控制水稻耐冷性相关性状的QTLs,其中,控制耐冷级别、叶枯萎度及苗成活率的QTLs各有2个,而且每个性状的2个QTLs都分别定位在第8染色体的RM22772-C61344和第11染色体的M100-RM26567区域内,这些QTLs具有较高的耐冷表型贡献率（14.09%~28.60%）。QTL的定位结果采用置换检验（Permutation test）进行了验证,发现控制耐冷级别的2个QTLs的F值都超过了QTL检测的阈值,而叶枯萎度和苗成活率2个性状的QTLs中各有1个QTL（q LWR-8和q SR-11）的F值超过了QTL检测的阈值。研究结果显示在第8和11染色体确实存在2个与水稻耐冷性相关的位点,可为进一步的分析和精细定位打下基础,也为耐冷水稻品种育种和种质创新提供理论依据。A 137-individual F2 mapping population, which derived from the cross between Fujisaka 5 and Jiangxi Simiao, was used to preliminarily map the QTLs for cold tolerant-associated traits of rice in seedling stage. The statistical method, Inclusive Composite Interval Mapping（ICIM）, was applied to genome-wide detection of QTLs conferring rice seedling cold tolerant ability using cold tolerance scale, leaf wilting rate, and survival rate of seedling after cold treatment as indices. Two QTLs were identified for each trait at the similar position in chromosome 8（RM22772-C61344） and chromosome 11（M100-RM26567）, respectively. The contribution of the QTLs to the phenotypic variation were from 14.09 to 28.60%. The permutation test was performed to confirm the results of QTL mapping, and similar QTLs were detected on the same marker intervals. These findings lay a solid base for further fine mapping of these important cold tolerance QTLs in the future.

关 键 词：水稻 苗期 耐冷性 QTL(数量性状基因座位) 定位分析 

分 类 号：S511[农业科学—作物学]