肉果秤锤树花粉活力测定方法  

Study on Detection Methods for Pollen Viability of Sinojackia sacocarpa L.Q.Luo

作　　者：伏秦超[1] 王娟[1] 张西玉[1] 刘超[1] 刘芳[1] 

机构地区：[1]乐山师范学院,峨眉山生物多样性保护与利用研究所,乐山614000

出　　处：《分子植物育种》2015年第5期1146-1150,共5页Molecular Plant Breeding

摘　　要：本研究以离体培养萌发法为参照,从TTC法、I2-KI法、过氧化物酶沉淀法和醋酸洋红法4种方法中筛选出适宜快速测定肉果秤锤树(Sinojackia sarcocarpa L.Q.Luo)花粉活力的方法。结果表明:肉果秤锤树花粉离体培养萌发的最适条件为23℃培养下15%蔗糖、0.01 mg/m L硼酸,萌发率达84.85%;过氧化物酶沉淀法、TTC法、I2-KI法和醋酸洋红法测定的萌发率分别为78.45%、0、100%和37.01%。过氧化物酶沉淀法测定结果与离体培养萌发法的测定结果最接近(p<0.01),是简单快速测定肉果秤锤树花粉活力的最适方法。本研究筛选出的花粉离体培养萌发条件和花粉活力快速测定方法,可为进一步开展肉果秤锤树育种工作提供理论依据与技术支持。We firstly investigated the optimum condition of Sinojackia sacocarpa L.Q.Luo＇s pollen germination under in vitro culture condition, and then recruited four different methods to detect the pollen viability, such as triphenyltetrazolium chloride（TTC）, iodine-iodide kalium（I2-KI）, peroxide enzyme and acidcarmine method, in comparison with in vitro pollen germination method. Our results showed that a culture medium containing 15%sucrose and 0.01 mg/m L boric acid had the best effect on in vitro pollen germination at 23℃, and the pollen germination rate was up to 84.85%. Furthermore, we found the detection of pollen viability using peroxide enzyme, TTC, I2-KI and acidcarmine method was 78.45%, 0, 100% and 37.01%, respectively; These data suggested that peroxide enzyme method provided a quicker and more convenient means compared to other three methods, because its result was close to true viability ratio and dyeing was fast and clear. This study selected the optional condition for the pollen germination in vitro culture and identified a fast method for detecting the pollen vitality, which should provide the theoretic foundation for breeding work.

关 键 词：肉果秤锤树 花粉活力测定 测定方法 

分 类 号：S686[农业科学—观赏园艺]