p53抑制剂PFT-α对犬博卡病毒感染致靶细胞病变的影响  

The Effects of p53 Inhibitor on Cytopathic Changes of Targeted Cells Induced by MVC Infection

作　　者：张薇[1] 何丽萍[1] 李建宁[1] 孙玉宁[1] 

机构地区：[1]宁夏医科大学基础医学院,银川750004

出　　处：《宁夏医科大学学报》2015年第1期9-13,F0002,共6页Journal of Ningxia Medical University

基　　金：国家级大学生创新创业训练计划项目(201210752007)

摘　　要：目的探讨p53抑制剂(pifithrin alpha,PFT-α)对犬博卡病毒(minute virus of Canine,MVC)感染致WRD细胞(walter reed canine,WRD)病变的影响。方法首先MVC感染WRD细胞72h后,DAPI染色并观察靶细胞的形态学变化;给予不同浓度梯度的PFT-α处理WRD细胞,MTS法检测PFT-α对WRD细胞的毒性作用,以确定PFT-α的安全使用剂量;利用安全剂量的PFT-α分别采取提前(MVC感染前1h)或同时与病毒感染靶细胞,MTS试剂法检测PFT-α加入的不同方式对博卡病毒MVC感染致靶细胞病变的影响;以不同MVC感染复数(multiplicity of infection,MOI)处理WRD细胞,观察PFT-α在安全剂量和最佳处理方式作用下对MVC感染致靶细胞WRD病变的影响。结果 MVC感染致WRD细胞发生明显的凋亡等病理性改变;20μmol·L-1的PFT-α浓度是WRD细胞的安全使用剂量;安全剂量的PFT-α加入能够明显提高受感染细胞的细胞活力,与对应感染组相比差异均有统计学意义(P<0.05或P<0.01)。结论 PFT-α能够降低犬博卡病毒感染致WRD细胞病变的程度。Objective To investigate the effects of p53 inhibitor（ pifithrin alpha,PFT- α） on cytopathic changes of WRD cells induced by MVC infection. Methods Firstly,WRD were infected by MVC,and cell morphological change was detected by DAPI staining after 72 hours. In order to ascertain the security dosage of PFT- α,WRD cells were incubated with various concentration of PFT- α,and the cytotoxic effect PFT-αwas detected by MTS assay; Then WRD cells were induced by MVC infection,combined with the security dosage of PFT- αin advance or at the same time,which was to investigate the effect of treating way of PFT-αby using MTS assay. Finally,the effect of PFT- α,at security dosage,on cytopathic effects of targeting cells induced by various concentration of MVC infection was detected. Results Significantly cytopathic effects were found after MVC infected WRD;. The concentrations of PFT- α 20 μmol·L- 1were safe dose for WRD cells. Compared with the MVC infection group,in the safe dose of PFT- α,the viability of infected cells could be significantly improved（ P〈0. 05 or P〈0. 01）. Conclusion p53 inhibitor（ PFT- α） could decrease cell lesion severity to some extent after target cells were infected by MVC virus.

关 键 词：p53抑制剂 博卡病毒 WRD细胞 细胞病变 

分 类 号：R373[医药卫生—病原生物学]