油酸致大鼠急性肺损伤后肾脏功能及其病理变化的研究  

Research of Kidney Function and Pathological Change in Rats after Oleic Acid induced Acute Lung Injury

作　　者：邵萍[1,2] 付路[3] 张娜[4] 马申懋 马希刚[5] 常湘辉[2] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学第二附属医院呼吸内科,银川750001 [3]鞍钢集团总医院NICU,鞍山114001 [4]河北省人民医院重症医学科,石家庄050051 [5]宁夏医科大学总医院重症医学科,银川750004

出　　处：《宁夏医科大学学报》2015年第3期260-263,F0002,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81260583);宁夏自然科学基金(NZ10139)

摘　　要：目的观察油酸致大鼠急性肺损伤(ALI)后肾脏功能及其病理变化。方法 16只雄性SD大鼠随机分为对照组和ALI组,每组8只。ALI组尾静脉注射油酸(0.04m L·kg-1)复制ALI模型,对照组注射等剂量生理盐水,两组6h后处死取材,腹主动脉取血测定尿素氮、肌酐;同时采集大鼠肺组织,测定肺组织湿/干比(W/D);采集静脉血清、支气管肺泡灌洗液(BALF),采用BCA(bicinchonininc acid)蛋白检测法测定肺通透指数(LPI);观察肾脏大体标本变化,肺、肾脏组织行石蜡切片HE染色,光镜下观察肾组织病理学改变,进行肾小管损伤评分并与肺水肿指标做相关分析。结果 ALI组W/D比值和LPI明显高于对照组(P<0.01);光镜观察ALI组大鼠肺泡壁不完整,肺泡腔内大量炎性细胞浸润,红细胞渗出,肺泡间质水肿。ALI组较对照组血Scr和BUN明显增高(P<0.01);大体标本对照组肾脏组织未见明显异常,ALI组轻度肿胀、略增大,包膜紧张,光镜可见肾小球淤血,肾间质水肿,大量炎细胞浸润,肾小管上皮细胞水肿;ALI组肾小管损伤评分较对照组明显升高(P<0.01);ALI组肺水肿指标与肾小管损伤评分及血BUN、Cr水平成正相关(P<0.01)。结论油酸致大鼠ALI6h可引起急性肾损伤,以肾小管水肿为其特征性病理变化,且与ALI程度密切相关。Objective To observe the changes of kidney function and pathological of rats during ALI by oleic acid. Methods Sixteen healthy SD rats were randomly divided into control group and ALI group,each group with eight. Rats in ALI group were induced by intravenous injection of oleic acid（ 0. 04 m L·kg- 1） while rats in the control group were given normal saline（ 0. 04 m L·kg- 1） by IV. The animals were killed at 6h after intravenous oleic acid. Blood samples were collected from abdominal aorta immediately before the animals were killed for determination of Serum creatine（ Scr） and blood urine nitrogen（ BUN）. All animals were sacrificed to collect lung tissue of rats to test lungtissue wet / dry weight ratio（ W / D） and to collect rat blood serum,bronchoa- lvelar lavage finid to test lung permeability index（ LPI）. To observe the changes of renal general appearance,Lung and renal tissues were embedded by paraffin for HE staining and the pathologic structure of lung and kidney was observed under the optical microscope and kidney injury score were measured. Results The LPI and W / D of ALI group was significantly higher than that of control group（ P〈0. 01）. Serum creatine and blood urine nitrogen were higher significantly than that of the control group（ P〈0. 01）. Through general observation,the kidney tissue was normal in control group. In ALI group,the Kidney tissue was swelled slightly and capsular tensioned. Under the microscope,the alveolar wall was not complete in ALI group and there were lots of inflammatory cells infiltrating,red blood cell exudating and alveolar interstitial edema. Glomerular was bleeding widespread,renal interstitial edema and there were lots of inflammatory cells infiltrating,epithelium cells of renal tubular was edema. Compared to control group,the score of histological evaluation increased significantly after ALI（ P〈0. 01）. In ALI group,pulmonary edema index was significantly positively correlated with BUN,Scr and renal tubular damage score（ P〈0.

关 键 词：油酸 急性肺损伤 急性肾损伤 病理 

分 类 号：R563.8[医药卫生—呼吸系统]