归芪补肾颗粒对足细胞损伤中nephrin、desmin表达的影响  

Effects of expression of nephrin and desmin on podocyte adriamycin damage by advanced Guiqibushen Granules

作　　者：马威[1] 郑小利[2] 易艳东[3] 谢岱[3] 

机构地区：[1]武汉市第一医院中心实验室,湖北武汉430022 [2]武汉科技大学医院内科,湖北武汉430060 [3]武汉市第一医院药学部,湖北武汉430022

出　　处：《中国医院药学杂志》2016年第1期24-28,共5页Chinese Journal of Hospital Pharmacy

摘　　要：目的:探讨归芪补肾颗粒对阿霉素导致的足细胞损伤的保护作用。方法:以0.5μg·mL^(-1)阿霉素复制足细胞损伤模型,再分别以低、中、高剂量归芪补肾颗粒含药血清(2%,4%,8%)干预24 h后,分别检测各组细胞nephrin、p-cadherin、desmin、α-SMA mRNA(RT-PCR法)和蛋白(Western Blot法)表达。结果:RT-PCR结果显示:与空白对照组(C组)比较,阿霉素模型组(Model)nephrin、P-cadherin mRNA表达(0.342±0.140,0.094±0.012)显著降低(P=0.000),desmin、α-SMA mRNA表达(0.517±0.096,1.242±0.198)显著增高(P=0.001,0.000);与模型组比较,归芪补肾颗粒中(M-GQBS)、高剂量组(H-GQBS)nephrin、P-cadherin mRNA表达(0.536±0.086,0.254±0.060,0.546±0.113,0.274±0.069)显著降低(P=0.007,0.005,0.001,0.000),desmin、α-SMA mRNA表达(0.393±0.084,0.819±0.067,0.375±0.084,0.764±0.125)显著增高(P=0.024,0.010,0.000,0.000)中、高剂量组之间没有差异(P>0.05)。Western Blot结果显示,model组nephrin及Pcadherin蛋白含量显著降低(P=0.000),demin、α-SMA蛋白含量显著增高(P=0.000);与Model组比较,M-GQBS及HGQBS组nephrin、P-cadherin蛋白表达显著增高(P=0.002,0.003,0.005,0.002),demin、α-SMA含量显著降低(P=0.000)。结论:归芪补肾颗粒能减轻阿霉素引起的足细胞损伤,减缓足细胞上皮-间充质转分化。OBJECTIVE To investigate protective effects of Guiqibushen Granules on podocyte damaged by Adriamycin.METHODS A model of podocyte damage induced by 0.5μg/mL Adriamycin was established,and then intervened 24 h later by low,medium,or high doses of Guiqibushen granules containing serum（2%,4%,8%）.Expressions of mRNA（RT-PCR）and proteins（Western Blot）of nephrin,P-cadherin,desmin andα-SMA in each group were detected.RESULTS RT-PCR results showed that,compared with control group（C group）,expressions of nephrin,P-cadherin mRNA（0.342±0.140,0.094,0.012）in Adriamycin model group（Model group）decreased significantly（P=0.000）,and expressions of desmin,α-SMA mRNA（0.517±0.096,1.242±0.198）increased obviously（P=0.001,0.000）.Compared with model group,expressions of nephrin and P-cadherin mRNA in medium and high doses of Guiqibushen granules（M-GQBS,H-GQBS）（0.536±0.086,0.254±0.060,0.546±0.113,0.274±0.069）decreased significantly（P=0.007,0.005,0.001,0.000）,and desmin andα-SMA mRNA（0.393±0.084,0.819±0.067,0.375±0.084,0.764±0.125）increased significantly（P=0.024,0.010,0.000,0.000）.No difference was observed between middle and high dose groups（P〉0.05）.WB results showed that,compared with C group,expressions of nephrin and P-cadherin protein decreased significantly（P=0.000）,and demin andα-SMA increased obviously（P=0.000）.Compared with model group,nephrin and P-cadherin protein of M-GQBS,H-GQBS increased significantly（P=0.002,0.003,0.005,0.002）,and desmin andα-SMA decreased significantly（P=0.000）.CONCLUSION Guiqibushen granules can reduce podocyte damage induced by adriamycin,and relieve epithelial-mesenchymal-transition（EMT）of podocyte.

关 键 词：归芪补肾颗粒 足细胞 NEPHRIN DESMIN 

分 类 号：R96[医药卫生—药理学]