酪蛋白磷酸肽-无定形磷酸钙再矿化脱矿牙本质的效能  

Effect of casein phosphopeptide-amorphous calcium phosphate on remineralization of demineralized dentine

作　　者：唐成芳[1,2] 窦祈 刘瑞瑞[2,4] 沈丽娟[2] 柴治国 方明 陈吉华 

机构地区：[1]西安医学院口腔医学系,西安710021 [2]第四军医大学口腔医院修复科,军事口腔医学国家重点实验室,陕西省口腔医学重点实验室,西安710032 [3]济南军区青岛第一疗养院口腔科,山东青岛266071 [4]西安交通大学口腔医院修复科,西安710004

出　　处：《第三军医大学学报》2016年第2期173-177,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金重点项目(81130078,81000458);长江学者和创新团队发展计划(IRT13051);陕西省科学技术厅-社会发展科技攻关项目(2015SF242)

摘　　要：目的研究酪蛋白磷酸肽-无定形磷酸钙（casein phosphopeptide-amorphous calcium phosphate,CPP-ACP）体外再矿化脱矿牙本质的效能和机制。方法 1制备牙本质片20片,37%磷酸脱矿30s,随机数字表法分为2组,分别经人工体液（simulated body fluid,SBF）和CPP-ACP处理15 d之后进行显微硬度测定、表面形貌观察及X线衍射分析。2构建胶原纤维镍钛网,采用透射电镜观察CPP-ACP和SBF处理的重组胶原,并进行选区衍射分析。结果处理15 d后CPP-ACP组和SBF组牙本质表面显微硬度分别为（47.92±4.25）和（42.72±4.65）维氏硬度值,CPP-ACP组显著高于SBF组（P〈0.05）,且扫描电镜见CPP-ACP组牙本质表面较SBF组明显致密,有大量以羟基磷灰石为主的沉积物生成。透射电镜见CPP-ACP组重组胶原纤维上及周围分布有密集呈梭形的长50-60 nm的无定形磷酸钙。SBF组仅见少量类圆形无定形磷酸钙。结论 CPP-ACP可促进离体脱矿牙本质再矿化,表面形成以羟基磷灰石为主的矿物质沉积,可用于根面龋和牙本质过敏的非手术治疗。Objective To investigate the effect and mechanism of casein phosphopeptide-amorphous calcium phosphate（ CPP-ACP） on the remineralization of demineralized dentine,and provide reference for the prevention or non-invasive intervention of root caries and dentine hypersensitivity. Methods Twenty dentine specimens demineralized with 37% phosphoric acid gel for 30 s were randomly divided in 2 groups with a random digital table. Then the specimens were treated with simulated body fluid（ SBF） and CPP-ACP,respectively, for 15 d. The dentine surfaces were subjected to microhardness measurement,micromorphological evaluation and X-ray diffraction analysis. Collagen fiber nickel-titanium meshes were constructed and treated with SBF and CPP-ACP for 1 d. Then,the meshes were subjected to micromorphological observation and selected area diffraction analysis by transmission electron microscopy. Results The Vickers hardness values of the dentine surfaces in the CPP-ACP group and SBF group were 47. 92 ± 4. 25 and 42. 72 ±4. 65,respectively,and the microhardness in the CPP-ACP group was significantly higher than that in the SBF group after treatment for 15 d（ P〈0. 05）. The dentine surface in the CPP-ACP group was more compact than that in the SBF group,and many sediments were generated on the dentine surface and identified mainly as hydroxylapatite（ HA）. Lots of 50 - 60 nm fusiform ACP was distributed on the recombinant collagen fibers and the periphery in the CPP-ACP group,and only a few of round ACP was observed in the SBF group by transmission electron microscopy. Conclusion CPP-ACP can promote in vitro remineralization of demineralized dentine,and generate HA as main minerals on the dentine surface. So it can be used for the non-operative treatment of root caries and dentin hypersensitivity.

关 键 词：酪蛋白磷酸肽-磷酸钙 牙本质 再矿化 牙本质过敏 根面龋 

分 类 号：R318.08[医药卫生—生物医学工程]