子痫前期患者胎盘组织中HSP70和NF-κB的表达及意义  

Expression of HSP70 and NF-κB in the placental trophoblasts of pregnant women complicated with preeclampsia

作　　者：林培红[1] 胡继芬[1] 

机构地区：[1]福建医科大学附属福州市第一医院妇产科,福建福州350009

出　　处：《中国医药导报》2016年第2期61-64,共4页China Medical Herald

基　　金：福建省福州市科技计划项目(2014-S-136-6)

摘　　要：目的探讨子痫前期患者胎盘组织中HSP70和NF-κB的表达差异及意义。方法选取2013年12月～2015年1月在福建医科大学附属福州市第一医院住院分娩的65例孕妇为研究对象，其中正常妊娠25例（正常妊娠组），轻度子痫前期17例（轻度子痫前期组），重度子痫前期23例（重度子痫前期组）。采用免疫组化法及实时荧光定量PCR检测孕妇胎盘HSP70和NF-κB的表达，并分析HSP70与NF-κB的相关性。结果免疫组化结果提示：HSP70及NF-κB在正常妊娠组、轻度子痫前期组及重度子痫前期组患者胎盘组织中的染色程度依次增高，轻度及重度子痫前期组HSP70及NF-κB阳性表达率与正常妊娠组比较差异有高度统计学意义（P〈0．01），但轻度及重度子痫前期组之间差异无统计学意义（P〉0．05）；实时荧光定量PCR结果提示：与正常妊娠组比较，HSP70及NF-κB在轻度子痫前期组及轻度子痫前期组中的表达水平增高，轻度子痫前期组及重度子痫前期组与正常妊娠组两两比较差异有高度统计学意义（P〈0．01）。相关性分析结果显示，HSP70与NF-κB呈正相关（r＝0．692，P〈0．01）。结论子痫前期患者胎盘组织中HSP70和NF-κB表达增加，可能与子痫前期的发生、发展有关。Objective To investigate the difference and significance between the expression of HSP70 and NF-κB in the placenta tissues of normal pregnancy and preeclampsia patients, in order to find out the relation between HSP70 and NF-κB. Methods From December 2013 to January 2015, 65 pregnant patients in the First Affiliated Hospital of Fujian Medical University were selected. There were 25 healthy pregnant women （normal pregnant group）, 17 mild preeclampsia patients （mild preeclampsia group） and 23 severe preeclampsia patients （severe precclampsia group）. The expressions of HSP70 and NF-κB in the placental tissues were measured using immunohistochemistry methods and real-time fluorescence quantitative PCR technique. Results HSP70 and NF-κB immunohistochemical results suggested that the staining degree of the normal pregnant group, the mild and severe preeclampsia group increased gradually. The differences of the expressions of HSP70 and NF-κB between the mild, severe preeclampsia group and the normal pregnant group were significant （P 〈 0.01）, while there was no statistical difference between the mild preeclampsia group and the severe preeclampsia group （P 〉 0.05）; the results of HSPTO and NF-κB real-time fluorescence quantitative PCR suggested that, the level of the expression increased in mild preeclampsia group and severe preeclampsia group, as compared with the normal pregnant group, the difference between the mild preeclampsia group, severe preeclampsia group and the normal pregnant group was statistically significant （P 〈 0.01）. The results of correlation analysis showed that, the expression of HSP70 was positively correlated with NF-κB （r = 0.692, P 〈 0.01）. Conclusion The expression of HSP70 and NF-κB increases in placental tissues of patients with preeclampsia, and may be associated with the occurrence and development of preeclampsia.

关 键 词：子痫前期 胎盘组织 热休克蛋白70 核因子-ΚB 

分 类 号：R714.246[医药卫生—妇产科学]