玻璃-PDMS薄膜-玻璃夹心微流控芯片制作  

Fabrication of glass-PDMS film-glass sandwich microfluidic chip

作　　者：王丽[1] 李刚[2] 金庆辉[1] 赵建龙[1] 

机构地区：[1]中国科学院上海微系统与信息技术研究所传感技术国家重点实验室,上海200050 [2]重庆大学光电工程学院,重庆400044

出　　处：《传感器与微系统》2016年第1期101-103,共3页Transducer and Microsystem Technologies

基　　金：国家“973”计划资助项目(2012CB933303,2011CB707505);国家自然科学基金资助项目(61271161,61271162);中国科学院科研装备研制项目(YZ201143)

摘　　要：聚二甲基硅氧烷(PDMS)是目前微流控芯片中应用的最广泛的基质材料,但基于PDMS材料的微流控芯片在应用中存在溶剂易挥发、难以进行较长时间检测分析的问题。针对这些问题,发展了一种简便高效的"玻璃—PDMS薄膜—玻璃"(G—P—G)夹心式微流控芯片的制作方法。该方法巧妙利用聚酯(PET)胶片作为载体转移处理脆弱的结构化PDMS膜,然后通过等离子体处理将结构化PDMS膜夹在两个玻璃基板的中间,形成"玻璃—PDMS薄膜—玻璃"夹心结构芯片,由于玻璃材料的低通透性,使得芯片具有低溶剂挥发特性。该夹心式芯片的低溶剂挥发特性通过蛋白质结晶实验得到了验证。Polydimethylsiloxane（PDMS）is currently the most widely used substrate material in microfluidic chip, but, microfluidie chip with this material has some problems in application, such as volatile solvent and difficulty in long term testing. To solve these problems, a simple and high-efficient fabrication method is developed to make ＇ glass-PDMS film-glass＇ （G-P-G）sandwich structure microfluidic chip. This method uses polyester（PET） film as carrier to transfer and handle structured PDMS film, and then the PDMS film is sandwiched between two glasses through the plasma treatment, to form sandwich structure chip, it has the feature of low solvent evaporation because of low permeability of glass material. Low solvent evaporation characteristics of the sandwich chip is validated through protein crystallization experiment.

关 键 词：聚二甲基硅氧烷薄膜 夹心结构 微流控芯片 低溶剂蒸发 蛋白质结晶 生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]