绵羊BMPR-IB基因第7外显子多态性与产羔性状的相关性研究  

Correlation between polymorphism of BMPR-IB gene exon7 and lambing performance of sheep

作　　者：贾建磊[1] 张利平[1] 丁强[1] 杨志文[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《西北农林科技大学学报（自然科学版）》2016年第1期7-13,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：甘肃省农牧厅生物技术专项(GNSW-2012~24);国家自然科学基金(地方基金)项目(31260547)

摘　　要：【目的】探索绵羊BMPR-IB基因第7外显子多态性与产羔数的相关性,寻找控制绵羊繁殖力的分子标记位点。【方法】以相同饲养管理条件下同龄已有产羔记录的蒙古羊(单胎母羊和双胎母羊)、无角陶赛特羊(单胎母羊和双胎母羊)、小尾寒羊(多胎母羊)为试验材料,采用PCR-SSCP结合DNA直接测序的方法,对BMPR-IB基因第7外显子进行多态性检测与分析。【结果】试验羊只存在AA型和AB型2种基因型;AB型个体在BMPR-IB基因第864位点发生C→T突变,出现T/C的杂合,所有试验样本的优势基因型均为AA型,优势等位基因均为A基因。χ2适合性检验表明,无角陶赛特羊、蒙古羊和小尾寒羊均处于Hardy-weinberg平衡状态,BMPR-IB基因第7外显子不同基因型在不同产羔类型绵羊中的分布差异显著,最小二乘法分析表明,绵羊BMPR-IB第7外显子多态性与产羔数有一定的相关性。【结论】BMPR-IB基因第7外显子第864位点的突变在不同繁殖力母羊群体中分布不同,表明绵羊BMPR-IB基因第7外显子多态性与绵羊产羔数相关,可以作为控制绵羊繁殖力的潜在遗传标记位点进行下一步研究。[Objective] This study aimed to explore the correlation between polymorphism of BMPR- IB gene exon7 and lambing performance in sheep and find locus to control reproduction of sheep. [Method] The breeding ewes including Mongolia ewes,Small Tail Han ewes and Poll Dorest ewes with different lam- bing performances （e. g. multiples,twins and singletons） were selected for studying polymorphism of BM- PR-IB gene exon7 detected by PCR-SSCP and DNA sequencing. [Result] AA and AB genotypes were found in tested ewes. AB genotype had C-~T mutation in coding region of 864 with heterozygous T/C. Genotype AA was dominant genotype and allele A was dominant allele in all samples. All sheep were in Hardy-weinberg equilibrium based on ）~2 test. There were significant differences in distribution of different genotypes of BMPRIB gene exon7 in different sheep types. Least squares analysis showed that there was correlation between polymorphism of BMPR-IB gene exon7 and lambing performance. [Conclusion] There was correlation between polymorphism of BMPR-IB gene exon7 and lambing performance of sheep. Themutation in locus 846 of BMPR-IB gene exon7 could be considered as a potential locus to control reproduc- tion of sheep.

关 键 词：绵羊 BMPR-IB基因第7外显子 PCR-SSCP DNA直接测序 多态性 产羔数 

分 类 号：S826.2[农业科学—畜牧学]