家蚕肠道感染细菌后6种抗菌肽基因表达的变化  

Expression of 6 antimicrobial peptide genes in silkworm,Bombyx mori after oral bacterial infection

作　　者：王延稳[1] 吕志强[1] 

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2016年第1期185-191,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家重大基础研究发展计划(973计划)子课题(2012CB114604);教育部基本科研业务费专项(CX200908)

摘　　要：【目的】研究感染绿脓杆菌(Pseudomonas aeruginosa)和金黄色葡萄球菌(Staphyloccocus aureus)后,家蚕中肠和脂肪体中6种抗菌肽基因表达的变化,为家蚕肠道免疫研究提供一定的理论依据。【方法】给家蚕喂食感染绿脓杆菌和金黄色葡萄球菌,以喂生理盐水为对照,利用半定量RT-PCR方法,检测感染不同时间(1,2,4,8,16,24h)后中肠和脂肪体中6种抗菌肽基因表达的变化。【结果】喂食绿脓杆菌1,2h后,中肠中抗菌肽Gloverin2、Lebocin、CecropinB6、CecropinD和Moricin的相对表达量显著上调;喂食金黄色葡萄球菌1,2,4h后,中肠中6种抗菌肽的相对表达量均显著上调。在脂肪体中,感染两种细菌后,仅抗菌肽CecropinD、Moricin和Attacin2相对表达量的变化较为显著。【结论】家蚕在感染不同细菌后所诱导的免疫机制不同。细菌经由口器进入家蚕肠道,可同时引起上皮免疫反应和体液免疫反应。[Objective] The expression of 6 antimicrobial peptide genes after oral infection with Pseud- omonas aeruginosa and Staphylococcus aureus was studied to provide theoretical basis for gut immune of silkworm. [Method] Using physiological saline as control check, the expression of 6 antimicrobial peptide genes in midgut and fat body of silkworm after 1,2,4,8,16,and 24 h Pseudomonas aeruginosa and Staph- ylococcus aureus infection was studied by semi-quantitative RT-PCR. [Result] Silkworm significantly up- regulated the relative expression of Gloverin2,Lebocin,CecropinB6,CecropinD and Moricin after infection 1,2 h with P. aeruginosa and the relative expression of all 6 antimicrobial peptides increased after infection 1,2,and 4 h with S. aureus in midgut. After oral infection with two bacteria, the relative expression of CecropinD,Moricin and Attacin2 was significant different in fat body. [Conclusion] The immune mecha- nisms of silkworm against different bacteria were different. Bacteria in intestine via mouthparts could in duce both epithelial immune response and humoral immune response.

关 键 词：家蚕 细菌 抗菌肽基因 半定量RT-PCR 

分 类 号：S186[农业科学—农业基础科学] Q966[生物学—昆虫学]