腈菌唑分子印迹固相萃取柱的制备及其在饲料检测中的应用  

Preparation of myclobutanil molecularly imprinted solid extraction colum and its application in feed detection

作　　者：何丛薇 高林[1,2] 赵春娟[1,2] 高文惠[1,2] 

机构地区：[1]河北科技大学生物科学与工程学院,河北石家庄050000 [2]河北省发酵工程技术研究中心,河北石家庄050018

出　　处：《中国饲料》2016年第1期24-27,共4页China Feed

基　　金：河北省科技支撑计划项目(14227504D、14236602D-13)

摘　　要：本实验在制备腈菌唑分子印迹固相萃取柱的基础上，建立了分子印迹固相萃取-高效液相色谱检测饲料样品中腈菌唑残留的方法。以腈菌唑为模板分子，运用本体聚合技术制备分子印迹固相萃取剂，装填成柱，使用该柱对饲料样品进行萃取净化，并采用高效液相色谱法对样品中腈菌唑残留进行检测。实验对固相萃取柱的制备条件和分离富集条件以及检测条件进行了研究。结果表明，采用本方法对样品处理选择性强，样品净化效果好；腈菌唑线性范围为o．3～200μg／mL，平均回收率为88．6％～96．5％．相对标准偏差（RSDs）为1．54％～3．02％（n=5）。该方法能高选择性、灵敏、快速、准确地检测样品中腈菌唑残留。On the basis of preparation of myclobutanil molecularly imprinted solid phase extraction column, a method for determination of myclobutanil residues in feed samples was established by molecularly imprinted solid phase extraction- high performance liquid ehromatography.Myclobutanil molecularly imprinted solid phase extraction agent was synthesized by bulk polymerization using myclobutanil as template molecule,and used to prepare myclobutanil molecularly imprinted solid phase extraction column.The extraction column was used to enrich myclobutanil in samples and cleanse samples, the cleaned samples were detected by high performance liquid chromatography.The selection of the extraction column preparation conditions,separation and enrichment conditions and detection conditions were investigated.The result showed that the method has good cleaning sample effect and strong selectivity, the linear range of myclobutanil was 0.3 200 μg/mL, the average recoveries were 88.6% - 96.5%,the relative standard deviations （RSDs）were 1.54% - 3.02% （n= 5 ）.The method was highly selective, sensitive, fast and accurate for determination of myclobutanil residues in samples.

关 键 词：饲料 腈菌唑 分子印迹聚合物 固相萃取柱 高效液相色谱法 

分 类 号：S816.17[农业科学—饲料科学]