不同钾和硼水平对棉花叶片逆境生理及其细胞壁硼的影响  

Effect of Different Levels of Potassium and Boron on Stress Physiology and Cell Wall Boron Content of Cotton Leaf

作　　者：吴秀文[1,2] 郝艳淑[1,2] 雷晶[1,2] 姜存仓[1,2] 

机构地区：[1]华中农业大学微量元素研究中心,湖北武汉430070 [2]农业部长江中下游耕地保育重点实验室,湖北武汉430070

出　　处：《农业资源与环境学报》2016年第1期29-34,共6页Journal of Agricultural Resources and Environment

基　　金：国家自然科学基金(40801112);公益性行业(农业)科研专项(201203013)

摘　　要：为了揭示钾、硼对棉花叶片细胞膜及硼利用的影响,本试验以棉花作为研究材料,采用营养液培养方式,研究了不同钾、硼处理叶片逆境下细胞膜及与细胞壁结合的硼比例的变化。结果表明,施钾水平为20 mg·L^(-1)时,缺硼(0 mg B·L^(-1))阻碍棉花地上部生长和干物质的积累,且与正常硼(0.2 mg B·L^(-1))相比,叶片逆境生理指标均显著升高,束缚态硼相对含量、R值(半束缚态硼/自由态硼)、细胞壁提取率和叶片总细胞壁硼占叶片硼的比例均有所升高;施钾水平为2 mg·L^(-1)时,缺硼叶片逆境生理指标、自由态硼和半束缚态相对含量及R值较正常硼处理也均显著升高,但束缚态硼相对含量和叶片总细胞壁硼占叶片硼的比例降低。因此,适钾与低钾时,缺硼均会明显破坏细胞膜透性。适钾条件下,缺硼导致细胞壁增厚,在细胞壁与果胶多糖结合的硼的比例升高;而低钾时,缺硼对于叶片细胞壁提取率无显著影响,进入叶片细胞质的硼的比例升高,但与果胶多糖结合的硼的比例降低。To find out the effect of potassium（K） and boron （B） on cotton leaf cell membrane and B distribution and utilization, the membrane relative permeability, MDA, Pro, the content of free B, semi-bound B and bound B and the content of B in cell wall of cotton leaf were analyzed under different K levels with solution culture method in this study. The results showed that in normal K （20 mgK·L^-1）, B deficiency （0 mgB ·L^-1） hindered the normal growth and dry mass of shoots, in addition, the membrane relative permeability, the content of MDA and Pro significantly increased compared with the normal B（0.2 mgB·L^-1 ）, and the relative concentration of bound B, R value, cell wall material and the ratio of total cell wall B/leaf B increased by 10.32%, 21.28%; 31.35% and 31.35%, respectively. In contrast, under low K（2 mgK·L^-1） supply, B deficiency produced a very significant decrease in the relative concentration of bound B and the ratio of total cell wall B/leaf B. The above results showed that under either K-deficient or K-sufficient condition, B deficiency damaged the cotton leaf cell membrane and cell membrane permeability. In normal B-supplied plants, lacking of B induced more B into the cytoplasm, but increased the proportion of B that combined with the pectic polysaccharides in cell wall. However, under K-deficient treatment, the proportion of B cross-linked pectic polysaccharides in cell wall decreased.

关 键 词：棉花 钾 硼 逆境生理 细胞壁硼 

分 类 号：Q945.78[生物学—植物学]