电针“内关”对缺血性心肌损伤大鼠钠离子通道相关蛋白的影响  

Effects of electroacupuncture at“Neiguan”(PC 6)on sodium channel-related proteins in rats with ischemic myocardial injury

作　　者：卞镝[1] 田辉[1] 隋月皎[1] 刘玉丽[1] 曹锐[1] 李成林[1] 李宝岩[1] 

机构地区：[1]辽宁中医药大学针灸推拿学院,沈阳110847

出　　处：《中国针灸》2016年第1期64-68,共5页Chinese Acupuncture & Moxibustion

基　　金：国家重点基础研究发展计划(973计划)项目:2012CB518500

摘　　要：目的：探讨电针＂内关＂对缺血性心肌的保护机制,阐明经穴效应循经特异性在心脏器官水平的钠离子通道的响应模式及特征。方法：将60只SPF级雄性大鼠随机分为空白组、模型组、非经非穴组、内关组、列缺组,每组12只。除空白组外,余组皮下注射异丙肾上腺素制备心肌缺血模型。内关组、列缺组、非经非穴组大鼠分别给予相应穴位的疏密波电针治疗,频率为2 Hz/20Hz,强度为2~3mA,每次电针20min,每天1次,连续7d。空白组与模型组仅在每天治疗时抓取固定。应用Western-blot法检测各组大鼠心肌电压-门控钠离子通道alpha（α）亚单位（Nav 1.5）、蛋白酪氨酸激酶（protein tyrosine kinase,PTKs）和蛋白酪氨酸磷酸酶（protein tyrosine phosphatases,PTPs）的蛋白表达水平。结果：模型组Nav 1.5和PTKs蛋白表达水平明显低于空白组（均P〈0.01）,内关组和列缺组较模型组表达水平增加（均P〈0.01）,内关组Nav 1.5和PTKs蛋白表达水平高于列缺组（均P〈0.01）;模型组和非经非穴组PTPs的蛋白表达均明显高于空白组（均P〈0.01）,内关组和列缺组PTPs的蛋白表达明显下调,均较模型组低（均P〈0.01）,内关组下调水平优于列缺组,两组比较差异具有统计学意义（P〈0.05）。结论：电针＂内关＂可能通过下调PTPs、上调Nav 1.5、PTKs的蛋白表达水平实现对钠离子通道电流以及钙超载的调节,进而改善心肌缺血状态,为经穴效应循经特异性理论提供实验基础。Objective To explore the protective mechanism of electroacupuncture（EA）at＂Neiguan＂（PC 6）on ischemic myocardial injury,and to explain the response patterns and characteristics of the specific effect of acupoints along meridians in sodium channel in the level of cardiac organ.Methods A total of 60 SPF male rats were randomly divided into a blank group,a model group,a non-acupoint group,a Neiguan group and a Lieque group,12 cases in each one.Except the blank group,rats in the remaining group were treated with subcutaneous injection of isoprenaline to establish the model of myocardial ischemia.Rats in the Neiguan group,Lieque group and nonacupoint group were treated with EA,dilatational wave,with a frequency of 2Hz/20 Hz.The intensity was 2—3mA.The needles were retained for 20 min per time,once a day for consecutive 7days.In the blank group and control group,the rats were grasped and fixed at the treating time each day.The western-blot method was used to test the expression of voltage-gated sodium channel alpha subunit（Nav 1.5）,protein tyrosine kinase（PTKs）and protein tyrosine phosphatase（PTPs）.Results The expression of Nav 1.5and PTKs in the model group was lower than that in the blank group（both P〈0.01）;the expression in the Neiguan group and Lieque group was higher than that in the model group（all P〈0.01）;the expression of Nav 1.5and PTKs in the Neiguan group was higher than that in the Lieque group（both P〈0.01）.The expression of PTPs in the model group and non-acupoint group was higher than that in the blank group（both P〈0.01）;the expression of PTPs in the Neiguan group and Lieque group was significantly down-regulated,which was lower than the model group（both P〈0.01）;the down-regulation in the Neiguan group was significantly different from that in the Lieque group（P〈0.05）.Conclusion EA at＂Neiguan＂（PC 6）,by down-regulating the expression of PTPs,up-regulating the expression of Nav 1.5and PTKs,is likely to achieve the aim of regulation on sodium

关 键 词：心肌缺血 电针 穴 内关 电压门控钠离子通道α亚单位(Nav1.5) 蛋白酪氨酸激酶(PTKs) 蛋白酪氨酸磷酸酶(PTPs) 

分 类 号：R246.1[医药卫生—针灸推拿学]