蛋白酶激活受体-2激动剂对小鼠胃肠动力的影响  

Effect of protease-activated receptor-2 agonist on gastrointestinal motility in mice

作　　者：陈金梅[1] 刘彩红[1] 谢立群[1] 郑艳敏[1] 

机构地区：[1]武警后勤学院附属医院消化内科,天津300162

出　　处：《中华实用诊断与治疗杂志》2016年第1期39-42,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：武警后勤学院附属医院种子基金项目(FYM201213)

摘　　要：目的 探讨蛋白酶激活受体-2（proteinase-actived receptors-2,PAR-2）mRNA和蛋白在小鼠胃肠道各部位的表达情况及PAR-2激动剂对小鼠胃肠动力的影响。方法 130只BABL/c小鼠,禁食不禁水16~24h后,取10只小鼠胃、十二指肠、空肠及回肠组织,采用RT-PCR法检测各部位PAR-2 mRNA表达情况,采用免疫组织化学法检测各部位PAR-2蛋白表达情况。余120只小鼠随机分为6组,分别为对照组、抑氨肽酶素A对照组（抑氨肽酶素A组）、SLIGRL-NH_2 1μmol/kg＋抑氨肽酶素A组（SLIGRL-NH_2 1μmol/kg组）、SLIGRL-NH_2 2.5μmol/kg＋抑氨肽酶素A组（SLIGRL-NH_2 2.5μmol/kg组）、SLIGRL-NH_2 5μmol/kg＋抑氨肽酶素A组（SLIGRL-NH_2 5μmol/kg组）、LRGILS-NH_2 5μmol/kg＋抑氨肽酶素A组（LRGILS-NH_2 5μmol/kg组）,每组20只。各组分别腹腔注射实验分组的相应药物,随后迅速给予葡聚糖蓝2000 0.4 mL灌胃,测定并比较6组小鼠小肠推进比和胃内色素排空率。结果PAR-2在小鼠胃、十二指肠、空肠及回肠均有表达;SLIGRL-NH_22.5μmol/kg组和SLIGRL-NH_2 5μmol/kg组小鼠胃内色素排空率[（73.65±8.61）%、（74.81±8.58）%]和小肠推进比[（62.75±3.52）%、（66.38±4.92）%]明显高于对照组[（62.13±7.82）%、（42.32±4.02）%]、抑氨肽酶素A组[（61.72±8.32）%、（43.21±5.12）%]和SLIGRL-NH_2 1μmol/kg组[（61.92±8.96）、（41.73±3.98）%]（P〈0.05）,SLIGRL-NH_2 2.5μmol/kg组与SLIGRL-NH_25μmol/kg组比较差异无统计学意义（P〉0.05）;对照组、抑氨肽酶素A组、SLIGRL-NH_2 1μmol/kg组和LRGILS-NH_2 5μmol/kg组小肠推进比、胃内色素排空率两两比较差异均无统计学意义（P〉0.05）。结论 PAR-2在小鼠胃肠道中广泛表达,在调节消化道功能中起重要作用,激活后可明显提高小鼠的胃肠动力。Objective To observe the expressions of proteinase-activated receptors-2 （PAR-2） mRNA and protein in each part of the gastrointestinal tract and to investigate the effect of PAR-2 agonist on gastrointestinal motility in mice. Methods Ten mice were randomly chosen from 130 BABL/c mice and were taken the stomach, duodenum, jejunum and ileum tissues after 16- to 24-hour fasting. The expression of PAR-2 mRNA was detected by real time-PCR, and the expression of PAR-2 protein was examined by immunohistochemistry. The other 120 BABL/e mice were randomly divided into six groups： control group, amastain group, 1 μmol/kg SLIGRL-NH2 ＋ amastain group （1 μmol/kg SLIGRL-NH2 group）, 2.5 μmol/kg SLIGRL NH2 ＋ amastain group （2.5 μmol/kg SLIGRL-NH2 group）, 5 μmol/kg SLIGRL-NH2 ＋ amastain group （5 μmol/kg group SLIGRL-NH2 ）, and 5 vmol/kg LRGILS-NH2 ＋ amastain group （5 μmol/kg LRGILS- NH2 group）, with 20 mice in each group. The mice in each group received intraperitoneal injection, followed by gavage with 0.4 mL blue dextran 2000. The gastric emptying rates and intestinal propulsion rates were detected and compared among six groups. Results PAR 2 was expressed in mucous layer, submucous layer and muscular layer of stomach and duodenum, jejunum and ileum. The gastric emptying rates （（73. 65 ± 8. 61）%, （74. 81±8. 58）%） and intestinal propulsion rates （（62.75±3. 52） %, （66. 38± 4. 92） %） in 2. 5μmol/kg SLIGRL-NH2 and 5μmol/kg SLIGRL-NH2 groups were significantly higher than those in control group （ （ 62. 13 ± 7. 82） %, （42. 32 ± 4. 02） % ）, amastain group （（61.72±8.32）%, （43. 21±5.12） %） and 1 μmol/kg SLIGRL-NH2 group （（61. 92±8. 96） %, （41. 73±3. 98） %） （P〈0.05）. There were no significant differences in gastric emptying rate and intestinal propulsion rate between 2.5 μmol/kg SLIGRL-NH2 group and 5 μmol/kg SLIGRL-NH2 group （P〉0.05）. And there were no significant differences between each two groups in control gr

关 键 词：蛋白酶激活受体-2 SLIGRL-NH2 胃肠动力 小肠推进比 胃内色素排空率 小鼠 

分 类 号：R965[医药卫生—药理学]