复方利血平氢氯噻嗪片中三组分的HPLC法测定  

Determination of Three Components in Compound Reserpine and Hydrochlorothiazide Tablets by HPLC

作　　者：陈建琴 

机构地区：[1]南宁食品药品检验所,广西南宁530001

出　　处：《中国医药工业杂志》2016年第1期76-78,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了高效液相色谱法测定复方利血平氢氯噻嗪片中的氢氯噻嗪、维生素B6和甲基橙皮苷。采用Agilent Zorbax ODS色谱柱,以乙腈∶0.1%磷酸溶液（用三乙胺调至p H 2.7）（25∶75）为流动相,检测波长283 nm。氢氯噻嗪、维生素B6和甲基橙皮苷在4-80μg/ml范围内线性关系良好。平均回收率分别为100.46%、99.35%和99.75%,RSD分别为0.64%、0.52%和0.89%。An HPLC method was established for the determination of hydrochlorothiazide, vitamin B6 and methyl hesperdine in compound reserpine and hydrochlorothiazide tablets. An Agilent Zorbax ODS column was used, with the mobile phase of acetonitrile ： 0.1% phosphoric acid （adjusted to pH 2.7 with triethylamine） （25 ： 75）, at the detection wavelength of 283 nm. The calibration curves were linear in the range of 4-80 μg/ml for the above three components. Their average recoveries were 100.46 %, 99.35 % and 99.75 %, with RSDs of 0.64 %, 0.52 % and 0.89 %, respectively.

关 键 词：复方利血平氢氯噻嗪片 氢氯噻嗪 维生素B6 甲基橙皮苷 高效液相色谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]