河南省2009-2011年伤寒副伤寒沙门菌分子分型与耐药研究  

Characteristics of drug resistance and molecular type of Salmonella typhi and Salmonella paratyphi isolated in Henan province, 2009-2011

作　　者：赵嘉咏[1] 谢志强[1] 穆玉姣[1] 苏佳[1] 夏胜利[1] 黄学勇[1] 许汴利[1] 

机构地区：[1]河南省疾病预防控制中心,郑州450016

出　　处：《中华流行病学杂志》2016年第1期111-114,共4页Chinese Journal of Epidemiology

摘　　要：目的分析2009-2011年河南省伤寒副伤寒沙门菌临床分离株耐药状况与PFGE分型特征。方法根据PulseNet公布的伤寒副伤寒沙门菌PFGE分型操作规程与美国临床实验室标准协会（CLSI）沙门菌K—B法药敏测试方案，对2009-2011年分离自河南省监测哨点医院78株伤寒副伤寒沙门菌进行抗生素药敏测试与PFGE分子分型。结果78株伤寒副伤寒沙门菌对8类13种抗生素均有不同程度的耐药，62株为多重耐药菌株（79．5％），其中耐2～3种4株（5．1％），耐5。8种41株（52．6％），耐9～10种14株（17．9％），耐11。12种3株（3．8％）；菌株对头孢类、喹诺酮类等5类抗生素耐药率总体呈上升趋势。经Xba I酶切与PFGE后，获得14种带型，每种带型包含菌株数1～47株不等，相似度为66．03％～100．00％。结论2000-2011年河南省临床分离的伤寒副伤寒沙门菌耐药状况普遍比较严重，PFGE带型呈现多样性的同时又具有较显著的优势带型特点，部分带型与其对应的耐药谱具有一定的关联性和聚集性。Objective To investigate the antibiotic resistance and pulsed field gel electrophoresis （PFGE） patterns of clinical isolates of Salmonella （S.） typhi and S. paratyphi in Henan province during 2009-2011. Methods According to molecular typing and Salmonella K-B drug susceptibility test method published by international PulseNet bacterial infectious disease monitoring network and USA Clinical and Laboratory Standards Institute （CLSI）, the drug susceptibility and PFGE molecule characteristics of 78 S. typhi and S. paratyphi strains isolated from sentinel hospitals in Henan were analyzed. Results The 78 strains ofS. typhi and S. paratyphi were resistant to 13 kinds of antibiotics, in which 62 were multidrng resistant （79.5%）, 4 were resistant to 2-3 kinds of antibiotics （5.1%）, 41 were resistant to 5-8 kinds of antibiotics （52.6%）, 14 were resistant to 9-10 kinds of antibiotics （17.9%）, 3 were resistant to 11-12 kinds of antibiotics （3.8%）. The resistant rate to cephalosporins, quinolones and other 3 kinds of antibiotic showed an increase trends. Seventy two strains ofS. typhi and S. paratyphi could be divided 14 molecular patterns by digestion with Xba I and PFGE, each pattern contains 1-47 strains which shared the similarity of 66.03%-100.00%. Conclusions The drug resistance of clinical isolates of S. typhi and S. paratyphi was serious in Henan. The PFGE patterns showed diversity, but the predominant pattems could be still found. The PFGE patterns of some strains were associated with their drug resistance.

关 键 词：伤寒副伤寒沙门菌 脉冲场凝胶电泳 分子分型 药敏测试 

分 类 号：R446.5[医药卫生—诊断学]