丙型肝炎病毒感染患者血清中细胞因子含量的研究  

Levels of serum cytokines in patients with hepatitis B virus infections

作　　者：李庆方[1] 高爱荣 石庆凤[1] 崔燕平[1] 刘雪妮[1] 

机构地区：[1]聊城市人民医院感染病科,山东聊城252000 [2]聊城市第三人民医院妇产科,山东聊城252000

出　　处：《中华医院感染学杂志》2016年第2期286-288,共3页Chinese Journal of Nosocomiology

基　　金：山东省科技厅研究基金资助项目(201478551-03)

摘　　要：目的探讨丙型肝炎病毒感染患者血清中细胞因子IL-6、IL-12、IL-18的变化与丙型肝炎患者病程发展的相关性,以了解患者免疫状况,为疾病的诊治提供参考。方法选取2013年10月-2015年6月医院就诊的63例丙型肝炎病毒感染患者作为研究对象,按临床症状分为3组,无临床症状组20例,慢性丙型肝炎轻度组25例,慢性丙型肝炎中重度组18例,另选健康志愿者21人为正常对照组,采用酶联免疫吸附法ELISA测定血清中IL-6、IL-12及IL-18水平,全自动生化分析仪检测谷丙转氨酶(ALT)水平;采用SPSS16.0软件对数据进行统计分析。结果 IL-6、IL-12及IL-18水平轻度及中重度组显著高于对照组(P<0.05);无临床症状组相应细胞因子水平也有升高,但与对照组比较差异无统计学意义;各组慢性丙型肝炎患者IL-12/IL-6的值与对照组比较显著下降(P<0.05);但无临床症状组与对照组比较差异无统计学意义;慢性丙型肝炎患者的血清IL-12、IL-6、IL-18水平与ALT呈正相关,相关系数分别为0.534、0.583、0.549(P<0.05)。结论慢性丙型肝炎患者IL-12与IL-6比例失衡可能与丙型肝炎病毒感染的免疫发病机制相关,患者血清中IL-12、IL-6及IL-18细胞因子与肝脏炎症相关,水平越高肝脏炎症越严重。OBJECTIVE To explore the correlation between the change of serum cytokines of the patients with hepatitis C virus infections such as IL-6,IL-12,and IL-18 and the development of disease course and understand the immune state so as to provide guidance for clinical diagnosis and treatment.METHODS A total of 63 patients with hepatitis C virus infections who were treated in the hospitals from Oct 2013 to Jun 2015 were recruited as the study objects and divided into three groups according to the clinical symptoms：the asymptomatic group with 20 cases,the mild chronic hepatitis C group with 25 cases,and the moderate and severe chronic hepatitis C group with 18cases;furthermore,21 healthy volunteers were chosen as the control group.The levels of serum IL-6,IL-12,and IL-18 were determined by using enzyme-linked immunosorbent assay（ELISA）,the level of alanine aminotransferase（ALT）was detected by means of automatic biochemical analyzer,and the statistical analysis of data was performed with the use of SPSS16.0software.RESULTS The levels of IL-6,IL-12,and IL-18 of the mild group and the moderate and severe group were significantly higher than those of the control group（P〈0.05）.The levels of the corresponding cytokines of the asymptomatic group were also elevated,as compared with the control group,there was no significant difference.As compared with the control group,the ratio of IL-12/IL-6of the patients with chronic hepatitis C in each group was remarkably reduced（P〈0.05）,however,there was no significant difference between the asymptomatic group and the control group.The levels of serum IL-12,IL-6,and IL-18 of the patients with chronic hepatitis C were positively correlated with the ALT,and the correlation coefficients were0.534,0.583,and 0.549,respectively（P〈0.05）.CONCLUSIONThe imbalanced ration between IL-12 and IL-6of the patients with chronic hepatitis C may account for the immune pathogenesis of the hepatitis C virus infections;the levels of serum IL-12,IL-6,and IL-18 are associated with live

关 键 词：丙型肝炎 细胞因子 白细胞介素 

分 类 号：R512.63[医药卫生—内科学]