天然四倍体泥鳅雄核发育二倍体诱导条件优化  

Optimizer of induction androgenetic dipoid in natural tetraploid loach

作　　者：林忠乔 李雅娟[1] 张赛赛[1] 姜志强[1] 王伟[1] 王玉生[1] 

机构地区：[1]农业部北方海水增养殖重点实验室,大连海洋大学,辽宁大连116023

出　　处：《东北农业大学学报》2015年第12期58-64,共7页Journal of Northeast Agricultural University

基　　金：国家自然科学基金(31272650);大连海洋大学博士启动基金项目(017208)

摘　　要：为探索安全、易操作的鱼类雄核发育新途径。以天然四倍体泥鳅为父本,二倍体泥鳅为母本人工催产授精,冷休克诱导雄核发育二倍体。试验选用L9(34)设计,进行三因素三水平正交试验,受精后时间为A,设0、5和10 min;处理温度为B,设0、3和6℃;处理时间C,设45、60和75 min。染色体倍性鉴定采用单个胚胎染色体计数法。结果表明,最高雄核发育二倍体诱导率为63.33%;根据正交试验直观分析结果,得出诱导天然四倍体泥鳅雄核发育二倍体各因素最优水平组合:受精后5 min,处理温度3℃,处理时间60 min;影响雄核发育三因素主次顺序为:受精后时间→处理温度→处理时间。In order to explore a new safe, easy operated approach of ichthyic androgenotes, used natural tetraploid loach as male parent and diploid one as female parent, to fertilized and hasten parturition manually, and induced androgenetic diploid loach by cold-shock of eggs fertilized with diploid sperm in this study. The orthogonal experiment of three factors and three levels [L9（3^4）] was used. The time after fertilization was A： 0, 5, 10 min; processing temperature was represented as B： 0, 3, 6 ℃; processing time was represented as C： 45, 60, 75 min. The identification of chromosome ploidy used counting method of singe embryo chromosome. The highest induction rate of androgenote diploid was 63.33%; according to the result of orthogonal experimental, could know the optimal solution of induction androgenetic diploid loach with the combination of several factors： 5 minutes after fertilization, the oosperm was treated under 3 ℃ for 60 minutes. The three factors affect androgenesis could be ranked as follow： fertilization time, processing temperature and processing time.

关 键 词：天然四倍体泥鳅 冷休克 雄核发育二倍体 正交试验 

分 类 号：S966.4[农业科学—水产养殖] S917.4[农业科学—水产科学]