济生肾气汤联合骨髓间充质干细胞移植对肝硬化大鼠T淋巴细胞亚群及红细胞免疫功能的影响研究  

Effect of Jisheng Shenqi Decoction Combined With Bone Mesenchymal Stem Cells Transplantation on T-lymphocyte Subsets and Red Cell Immune Function of Rats With Liver Cirrhosis

作　　者：刘莹[1] 周晓玲[2] 谢胜[3] 侯秋科[2] 李泽鹏[2] 陈峭[2] 李灿[2] 韦金秀[2] 

机构地区：[1]广西中医药大学研究生学院,广西南宁市530001 [2]柳州市中医院 [3]广西中医药大学第一附属医院

出　　处：《中国全科医学》2015年第36期4501-4505,共5页Chinese General Practice

基　　金：广西科学研究与技术开发计划项目课题(桂科攻1298003-2-10)

摘　　要：目的探讨济生肾气汤联合骨髓间充质干细胞(BMSCs)移植对肝硬化大鼠T淋巴细胞亚群及红细胞免疫功能的影响。方法 2014年10-12月,采用随机数字表法将造模成功的30只肝硬化大鼠分为对照组和实验组,每组各15只。对照组采用单纯BMSCs移植治疗,实验组采用济生肾气汤联合BMSCs移植治疗。治疗前及治疗后1、2、3、4周检测两组大鼠T淋巴细胞亚群和红细胞免疫功能指标。结果 CD_4^+、CD_8^+、CD_4^+/CD_8^+比较,治疗方法与时间存在交互作用(P<0.05);组间比较,差异有统计学意义(P<0.05);时间间比较,差异有统计学意义(P<0.05)。其中,治疗前和治疗后1、2周对照组大鼠CD_4^+、CD_8^+、CD_4^+/CD_8^+与实验组比较,差异均无统计学意义(P>0.05);治疗后3、4周对照组大鼠CD_4^+、CD_8^+、CD_4^+/CD_8^+与实验组比较,差异均有统计学意义(P<0.05);对照组和实验组大鼠治疗后2、3、4周CD_4^+、CD_8^+、CD_4^+/CD_8^+与治疗前比较,差异均有统计学意义(P<0.05)。红细胞C3b受体花环率(RBC·C3bRR)、红细胞免疫复合物花环率(RBC·ICR)比较,治疗方法与时间存在交互作用(P<0.05);组间比较,差异有统计学意义(P<0.05);时间间比较,差异有统计学意义(P<0.05)。其中,治疗前和治疗后1、2周对照组大鼠RBC·C3bRR、RBC·ICR与实验组比较,差异均无统计学意义(P>0.05);治疗后3、4周对照组大鼠RBC·C3bRR、RBC·ICR与实验组比较,差异均有统计学意义(P<0.05);对照组和实验组大鼠治疗后3、4周RBC·C3bRR、RBC·ICR与治疗前比较,差异均有统计学意义(P<0.05)。结论与单纯BMSCs移植相比,济生肾气汤联合BMSCs移植可明显改善肝硬化大鼠的T淋巴细胞亚群和红细胞免疫功能的相关指标。Objective To investigate the influence of Jisheng Shenqi decoction combined with bone mesenchymal stem cells（BMSCs） transplantation on T-lymphocyte subsets and red cell immune function of rats with liver cirrhosis.Methods From October to December in 2014,liver cirrhosis mode was successfully built in 30 rats,and the rats were divided into control group and trial group using convenience number table method with 15 in each group.Control group was administrated with only BMSCs transplantation,and trial group was administrated with Jisheng Shenqi decoction combined with BMSCs transplantation.Before treatment and 1,2,3 and 4 weeks after treatment,the T- lymphocyte subsets and red blood cell immune function of the rats were measured.Results In the comparison of CD_4~＋,CD_8~＋ and CD_4~＋ /CD_8~＋,interaction effect existed between treatment method and time（P0.05）;significant differences existed between the two groups（P0.05）;significant differences existed between different time points（P〈0.05）.Before treatment,1 week and 2 weeks after treatment,control group were not significantly different（P0.05） from trial group in CD_4~＋,CD_8~＋ and CD_4~＋/CD_8~＋;3 weeks and 4 weeks after treatment,control group and trial group were significantly different（P〈0.05） in CD_4~＋,CD_8~＋ and CD_4~＋ /CD_8~＋;the CD_4~＋,CD_8~＋ and CD_4~＋ /CD_8~＋ of control group and trial group at 2 weeks,3 weeks and 4 weeks after treatment were significantly different from those before treatment（P〈0.05）.In the comparison of RBC ·C3bRR and RBC·ICR,interaction effect existed between treatment method and time（P〈0.05）;significant differences existed between the two groups（P〈0.05）;significant differences existed between different time points（P〈0.05）.Before treatment,1 week and 2 weeks after treatment,control group were not significantly different（P〉0.05） from trial group in RBC·C3bRR and RBC·ICR;3 weeks and 4 weeks after treatment,control group and trial group were significan

关 键 词：肝硬化 间质干细胞移植 济生肾气汤 T淋巴细胞亚群 模型 动物 

分 类 号：R657.31[医药卫生—外科学]