γ-L-谷氨酰-S-烯丙基-L-半胱氨酸对肝星状细胞线粒体功能的影响  

Effect of γ-L-glutamoyl-S-allyl-L-cysteine on Mitochondrial Function of Hepatic Stellate Cells

作　　者：张笔觅 孙瑞波[1] 吕智超[1] 杨明静[2] 李万良[2] 

机构地区：[1]吉林省经济管理干部学院,吉林长春130012 [2]吉林省农业科学院,吉林长春130033

出　　处：《安徽农业科学》2015年第35期220-222,255,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]通过研究γ-L-谷氨酰-S-烯丙基-L-半胱氨酸(GSAC)对大鼠肝星状细胞(HSC-T6)线粒体功能的影响,探讨GSAC对肝纤维化的抑制作用。[方法]利用微板法测定GSAC对培养24 h细胞线粒体代谢甲基噻唑基四唑(MTT)的影响,以罗丹明123作为染料,通过流式细胞仪检测线粒体膜电位。[结果]GSAC具有抑制HSC-T6线粒体代谢MTT的作用,IC50值为0.835 mg/ml;GSAC引起线粒体膜电位崩溃,所有GSAC处理组(0.2、0.4与0.8 mg/ml)罗丹明123荧光强度极显著高于对照组。[结论]GSAC可破坏HSC-T6细胞线粒体功能,提示其对肝纤维化具有抑制作用。[ Objective] The research aimed to explore inhibition effect of γ-L-glntamoyl-S-allyl-L-cysteine （GSAC） on liver fibrosis by studying the influence of GSAC on the mitoehondrial function of rat hepatic stellate cells （HSC-T6）. [ Method J After 24 h culture in different concentrations of GSAC, methyl thiazolyl tetrazolium （MTT） metabolism was tested, with the median inhibitory concentration （ IC50） value obtained in a microplate assay, and mitochondrial membrane potential （MMP） was tested in a flow cytometer assay with Rhodamine 123 as indicator. [Result]GSAC inhibited MTr metabolism, with IC50 =0.835 mg/ml, and GSAC inhibited MMP, the fluorescence intensity in GSAC treated groups （0.2, 0.4 and 0.8 mg/ml） significantly increased compared to the control. [ Conclusion] GSAC damages mitochondrial function of HSC-T6 cells, which suggests that GSAC has an inhibitory effect on liver fibrosis.

关 键 词：γ-L谷氨酰S烯丙基L半胱氨酸 肝星状细胞 线粒体膜电位 MTT 

分 类 号：S633.4[农业科学—蔬菜学]