海洋生防细菌在黄瓜根际土壤的定殖规律  

The colonization rule of marine biocontrol bacteria around cucumber The colonization rule of marine biocontrol bacteria around cucumber rhizosphere

作　　者：王淑芳[1] 汪晶晶[1] 王军强[1] 王琦[1] 马桂珍[1] 李世东[2] 孙漫红[2] 暴增海[1] 

机构地区：[1]淮海工学院海洋学院,连云港222005 [2]中国农业科学研究院植物保护研究所,北京100081

出　　处：《应用与环境生物学报》2015年第6期1095-1099,共5页Chinese Journal of Applied and Environmental Biology

基　　金：江苏省省属高校自然科学重大基础研究项目(12KJA210001);江苏省自然科学基金项目(BK20141248)资助~~

摘　　要：为揭示土壤初始pH值、温度和有机质含量三因素对多粘类芽孢杆菌(Paenibacillus polymyxa)L_1-9菌株在黄瓜根际土壤定殖的影响,采用室内盆栽实验,在黄瓜一叶期灌根接种生防菌L_1-9发酵液,设置土壤pH值、温度和有机质含量的不同处理,培养并定期采集黄瓜根际土壤样品,采用实时荧光定量PCR技术检测海洋生防菌L_1-9在黄瓜根际土壤定殖的数量变化.定量数据及其显著性检验结果表明:温度、初始pH值和有机质含量显著影响L_1-9菌株在黄瓜根际土壤中的定殖.土壤初始pH为6、7和8均可定殖.初始pH值为7和8的处理,在多数调查时间内无显著差异,最大为每克干土(8.52±0.18)×10~9拷贝.在存在显著性差异的时间段内,初始pH值为6时,L_1-9菌株在黄瓜根际土壤中数量在同期中最大.15-30℃培养时,最大数量为每克干土(2.13±0.31)×10^(10)拷贝.温度越高,定殖越早,但在较高温度下定殖数量下降较快.高有机质含量可以增加定殖数量,提高定殖能力,最大数量为每克干土(1.15±0.04)×10^(10)拷贝.本研究表明实时荧光定量PCR技术适用于研究土壤根际生防菌的定殖;海洋生防菌L_1-9能够在较宽温度、初始pH值和有机质含量范围内稳定定殖,较低温度、中性偏酸初始pH值和高有机质含量更有利于L_1-9菌株在黄瓜根际土壤中定殖.The objective of this paper was to reveal the effects of 3 soil environmental factors, including the initial pH value, the temperature and the organic matter contents, on Paenibacillus polymyxa L1-9 rhizosphere colonization. We monitored the changes of the L1-9 strain colonizing the rhizosphere soil of cucumber growing in pots using real-time f luorescence quantitative-PCR. At the one-leaf stage, cucumber was inoculated by the irrigation root method. And rhizosphere soil samples of cucumber were collected every 2 d, for a total of nine times. Then, we quantified the 16 S rRNA gene number of the L1-9 strain in the cucumber rhizosphere, and researched the colonization characteristics and analyzed the effects of the environmental factors on L1-9 colonization in the rhizosphere soil of cucumber. The results showed that real-time PCR was suitable for colonization studies, and that the initial pH, temperature and organic matter contents all significantly influenced the colonization within a certain period. The biocontrol agent L1-9 could colonize soil with an initial pH of 6, 7 and 8. There were no significant differences between pH 7 and 8 treatments for the majority of investigations. Among all pH treatments in the same period, pH 6 had the greatest number of L1-9 strain, significantly different from others, which was（8.52 ± 0.18） × 10^9 copies per gram dry soil. Biocontrol agent L1-9 could maintain greater number over a longer period at 15°C than at higher temperatures. Colonization started earlier but also decreased more rapidly with higher temperatures. Among all the temperature treatments, the greatest 16 S rRNA copies number of L1-9 strain was（2.13 ± 0.31） × 10^10 per gram dry soil. On the other hand, high organic matter content increased L1-9 numbers and improved colonization ability, and the greatest 16 S rRNA copies number of L1-9 strain was（1.15 ± 0.04） × 10^10 per gram dry soil. The results indicated that Paenibacillus polymyxa L1-9 strain can colonize the rhizosphere soil of cucumber in

关 键 词：生物防治 根际定殖 温度 pH值 有机质含量 多粘类芽孢杆菌 实时荧光定量PCR技术 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S154.3[农业科学—植物保护]