烟曲霉极性因子kipA基因的敲除与鉴定  

Identification and knocking-out of polarized growth factor gene kipAin Aspergillus fumigates

作　　者：朱均昊[1] 李莉 章强强[1] 陈裕充 

机构地区：[1]复旦大学附属华山医院皮肤科真菌室,上海200040 [2]上海市皮肤病医院皮肤外科,上海200050

出　　处：《中国真菌学杂志》2015年第6期332-335,共4页Chinese Journal of Mycology

基　　金：国家自然科学基金(81271762);高等学校博士学科点专项科研基金新教师类课题(20120071120069)

摘　　要：目的构建烟曲霉kipA基因突变株。方法通过In-Fusion技术将kipA基因的上下游基因与抗性基因顺序链接,以潮霉素抗性基因替换靶基因,达到用同源重组手段敲除烟曲霉kipA基因的目的,以realtime-PCR对基因突变株进行鉴定和验证。结果 RT-PCR结果显示,成功构建烟曲霉kipA基因突变株。结论烟曲霉kipA基因突变株的构建,为后续烟曲霉极性生长与血管侵袭性的研究提供了实验基础。Objective To construct the kipA mutant strain.Methods The vector containing upper and downstream genes of kipAlinked with hygromycin resistance gene in the middle was constrctied via In-Fusion assembly.The kipA gene of Aspergillus fumigatu was replaced with hygromycin resistance gene via the method of homologous recombination.The mutant strain was identified and validated by realtime-PCR.Results The kipA deletion strains were successfully constructed.Conclusions Successful construction of kipA mutant strain would provide the foundation for the research of polarized growth and vascular invasion of Aspergillus fumigatus.

关 键 词：烟曲霉 kipA基因 极性生长 

分 类 号：R379.6[医药卫生—病原生物学]