外翻肠囊法评价黄连-大黄药对不同提取工艺中生物碱的肠吸收特性  

Evaluation on intestinal absorption of alkaloids extracted by different methods from Rhizoma Coptidis-Rheum rhabarum herbal pair via everted gut sacs

作　　者：陈凯[1,2] 王月亮 陈彦[3] 李慧[2] 刘宇灵[2] 王佳奇[1] 张晓莉[2] 刘文丛[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]中国中医科学院中药研究所,北京100700 [3]江苏省中医药研究院中药组分与微生态研究中心,江苏南京210000

出　　处：《中国中药杂志》2015年第24期4853-4859,共7页China Journal of Chinese Materia Medica

基　　金：中国中医科学院自主选题项目(Z02063);2009年中医药行业科研专项(200907001-5)

摘　　要：采用水煎和醇提2种工艺,通过大鼠肠外翻模型评价黄连-大黄配伍药对中生物碱的体外肠吸收特征。以小檗碱、巴马汀、黄连碱、表小檗碱4种主要生物碱成分为代表,采用一测多评法对其进行定量分析,计算吸收速率常数,进而评价2种工艺中,4种生物碱成分在大鼠不同肠段的体外吸收特征。结果显示:2种不同工艺提取物中上述4种成分的高、中、低剂量在小肠段均为线性吸收,R^2大于0.99,符合零级吸收速率,小肠部位的吸收速率常数(K_a)表现为水煎组>醇提组。The research aimed to evaluate the intestinal absorption of alkaloids extracted by decoction and alcohol extraction processes from Rhizoma Coptidis-Rheum rhabarum herbal pair via everted gut sacs. Berberine, palmatine, coptisine and epiberberine were the main alkaloids in this herbal pair and taken as the standard indexes in the quantitative analysis with multi-components by single marker （QAMS） method, in order to calculate absorption rate constant （/~） and evaluate intestinal absorption characteristics of these four alkaloids extracted by different extraction methods in different intestinal segments in rats. The results showed that the four alkaloids extracted by two different processes in high, medium and low doses had linear absorption properties in the small intestine segment, which conformed to zero-order absorption rate, intestinal segment than 0.99. The absorption rate constant（K2） of decoction group was higher than that of alcohol extraction group.

关 键 词：黄连 大黄 肠外翻 吸收速率常数(Ka) 

分 类 号：R285.5[医药卫生—中药学]