陈皮“陈久者良”之黄酮类成分增加原因探究  

Study of reason of “the older,the better”of Pericarpium Citri Reticulatae(PCR) which contributed increase of flavonoids

作　　者：王福[1] 张鑫[1] 卢俊宇[1] 梅国荣[1] 陈鸿平[1] 陈林[1] 刘友平[1] 

机构地区：[1]成都中医药大学药学院中药材质量标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中国中药杂志》2015年第24期4890-4896,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81072991);国家基础科学人才培养基金项目(J1310034);川产大宗药材川陈皮快速无损模式识别及品质评价模型研究(2015JY0012)

摘　　要：研究陈皮"陈久者良"之黄酮类成分增加的原因。采用显微与分子鉴定方法对陈皮样品表面分离纯化真菌进行鉴定;应用HPLC对陈皮霉变前后芸香柚皮苷、陈皮苷、川陈皮素与桔皮素4种黄酮类成分的变化规律进行了定量研究;采用紫外分光光度法测定了陈皮霉变前后总黄酮含量变化;应用逆向思维对筛选出的真菌进行反接培养,筛选出与黄酮类物质变化密切相关菌株。最终从8批次陈皮样品中共分离鉴定出25株真菌,分属于2属5种,分别为普通青霉、朱黄青霉、桔青霉、黄曲霉和黑曲霉,同时,陈皮霉变后黄酮类成分含量增加,其中主要影响黄酮类成分增加的菌株为黑曲霉。因此,陈皮"陈久者良"有一定的科学道理,其黄酮类成分的增加与表面污染黑曲霉等真菌的代谢活动相关。This study is to explore the reason of ＂the older, the better＂ of PCR and itsincrease offlavonoids. We identified the, fungus isolated from the PCR using microscopic and molecular identification. HPLC method was used to determine the content of 4 flavonoids and to clarifythe regularity of them ; UV spectrophotometty method was used to determine the total content of flavonoids ; reverse thinking was applied to screen the fungus that have close relation to the change of flavonoids. Finally, we have isolated and identified 25 fungusfrom the PCR, which belong to 2 genus and 4 species, including pencillium commune, P. minioluteeum, P. citrinum, Aspergillus flavus and A. niger. The content of flavonoids was increased in the mildew PCR due to A. niger and other fungus. Therefore, ＂the older, the better＂ of PCR had its scientific reason that the increase of flavonoids had a close relation of the metabolic, activity of A. niger and other fungus.

关 键 词：陈皮 霉变 陈久者良 真菌 黄酮 

分 类 号：R284[医药卫生—中药学]