慢性阻塞性肺疾病大鼠支气管肺泡灌洗液中CD80、CD86的变化及意义  

The role of CD80 and CD86 in rats with chronic obstructive pulmonary disease

作　　者：顾延会[1] 符丹丹[1] 饶习敏[1] 欧阳瑶[1] 

机构地区：[1]遵义医学院附属医院呼吸内科,贵州遵义563099

出　　处：《遵义医学院学报》2015年第6期595-598,共4页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:81460008)

摘　　要：目的研究树突状细胞(DCs)表面因子CD80、CD86在慢性阻塞性肺疾病(COPD)大鼠造模过程中不同时间段支气管肺泡灌洗液(BALF)中的表达变化,进而深入探讨DCs在COPD发病过程中的作用。方法将48只大鼠随机分为正常对照组(n=24)和COPD模型组(n=24)。COPD模型组大鼠4周内间断予气道内注入脂多糖(LPS)溶液2次,并予被动吸烟2次/日(注入脂多糖当天除外)。两组大鼠分别于第7、14、21及28天随机选取6只,取其肺组织行病理苏木精-伊红染色(HE染色)以观察病理形态学改变,同时收集BALF,采用流式细胞技术(FCM)检测两组大鼠4个时间段BALF中CD80、CD86的百分比表达。结果随时间进展COPD大鼠气道炎症和肺气肿表现逐渐出现,且日渐加重,第28天显示明显气道阻塞和肺气肿表现。正常对照组大鼠BALF中CD80和CD86百分比在第7、14、21和28天无明显变化,差异无统计学意义。与正常对照组同时间段相比,COPD模型组第7、14、21、28天BALF中CD80和CD86百分比均有所增加,但第7、14天增加不明显,差异无统计学意义,第21、28天增加明显,差异有统计学意义。结论利用烟熏及气道内注LPS方法 4周后成功建立大鼠COPD模型。COPD模型组大鼠造模早期、中期BALF中CD80、CD86百分比表达不明显,后期增加明显,提示DCs可能随疾病进展逐渐参与到COPD的炎症反应过程中,并导致后期不可逆的疾病进展,同时提示早期预防和治疗可能减少免疫反应引起的炎症反应并阻止疾病进一步进展。Objective To study the expression changes of CD80 and CD86,as the costimulatory molecules of dendritic cell（DCs）,in the bronchoalveolar lavage fluid（BALF）of Chronic Obstructive Pulmonary Disease（COPD）at different time periods in rats,and the effect of DCs in this pathogenesis.Methods 48 rats were randomly divided into control group（n = 24）and COPD model group（n = 24）.The rats of COPD model group were established via intra-tracheal injection of lipopolysaccharide（LPS）solution twice in 4 weeks and cigarette inhalation twice daily.Then lung tissue and BALF of rats were investigated at 7-day,14-day,21-day and28-day,respectively,Lung tissue pathology was examined by hematoxylin-eosin（HE staining）,while the percentage of CD80 and CD86 was determined by FCM in the BALF.Results There was severe gradually inflammation in the airway and emphysema in COPD model,and the changes of pathology at 28-day were the same as those of COPD patients.There were no significant changes of CD80 and CD86 in BLAF at 7-day,14-day,21-day and 28-day in the control groups.Compared with the control group,the percentages of CD80 and CD86 in BALF were increased in COPD groups,but there were no significant increase at 7-day and 14-day,and significantly increased at 21-day and 28-day.Conclusion After four weeks,the COPD rat models were established successfully by this method.The percentages of CD80 and CD86 in BALF of COPD rat model groups were significantly upregulated,especially at 21-day and 28-day,suggesting that DCs may be gradually involved in COPD for inflammatory reaction,leading to the progression and irreversible COPD later.Early prevention and prompt treatment may reduce inflammation caused by the immune response and prevent further disease progression.

关 键 词：慢性阻塞性肺疾病 树突状细胞 肺泡灌洗液 CD80 CD86 

分 类 号：R563.3[医药卫生—呼吸系统]