茜素黄R与溶菌酶作用方式的光谱法研究  

Study on the interaction between alizarin yellow R and lysozyme by spectrometry

作　　者：马越[1] 欧亮龙[2] 王兴明[3] 

机构地区：[1]洛阳轴研科技股份有限公司,河南洛阳471039 [2]洛阳轴承研究所有限公司,河南洛阳471039 [3]西南科技大学材料科学与工程学院化学系,四川绵阳621010

出　　处：《商丘师范学院学报》2015年第12期41-45,共5页Journal of Shangqiu Normal University

摘　　要：在接近生理条件下,采用UV-Vis光谱、荧光光谱、热力学等方法研究了有机小分子茜素黄R(AYR)与溶菌酶(Lys)的作用机理.研究表明,AYR与Lys作用摩尔比为AYR∶Lys=5∶1,摩尔吸光系数εAL=3.87×10~5L·mol^(-1)·cm^(-1),说明AYR对Lys具有荧光猝灭特性,作用模式为静态猝灭,通过热力学实验可知,两者之间的作用方式主要是疏水作用和范德华力作用.The mechanism of interaction between alizarin yellow R and lysozyme has been investigated by UV spectroscopy,fluorescence spectroscopy,thermodynamics,etc. The results indicate that the molar ratio of AYR and Lys is 5∶ 1,the molar absorption coefficient is εAL= 3. 87 × 10^5L·mol^-1·cm^-1. The action type of AYR and Lys is static quenching. The thermodynamic experiment shows that the main effect of is hydrophobic effect and van der Waals force.

关 键 词：茜素黄R 溶菌酶 作用机制 

分 类 号：O657.3[理学—分析化学]