棘霉素对K562细胞生长及相关激酶的影响  

Effect of Echinomycin on Growth of K562 Cell Line and Related Kinases

作　　者：杜钢军[1] 许严伟 林海红[1] 

机构地区：[1]河南大学药学院,河南开封475004 [2]洛阳市第三人民医院药剂科,河南洛阳471002

出　　处：《河南大学学报（医学版）》2015年第4期229-231,235,共4页Journal of Henan University:Medical Science

基　　金：国家自然科学基金资助项目(30472082)

摘　　要：目的研究棘霉素对人白血病细胞K562生长及相关激酶的影响。方法取对数生长期的K562细胞,采用0.05、0.1、0.2、0.5、1.0、3.0μg/L棘霉素分别处理24h,MTT法检测细胞存活率,流式细胞术观察细胞周期分布,Western blot检测Bcr-Abl、AKT的表达。结果棘霉素对K562细胞的IC50为0.82μg/L。随着浓度的增加,棘霉素对酪氨酸激酶的抑制作用增强;棘霉素在2.0μg/L时,其抑制细胞蛋白激酶B的磷酸化程度最高。结论棘霉素可以抑制K562细胞的增殖,其机制与抑制酪氨酸激酶和下调蛋白激酶B的作用有关。Objective To explore the effect of echinomycin on the growth of K562 cell.Methods In experiments,K562 cells were treated with different concentrations（0.05,0.1,0.2,0.5,1.0,3.0μg/L）,and the cell proliferation was determined by MTT assay.The cell cycle,Bcl-Abl and AKT were examined by FACS,Western blot methods,respectively.Results The survival rate of K562 cells decreased gradually as the increase of echinomycin concentration,and the IC50 was 0.82μg/L.With the increase of concentration,echinomycin dose-dependently inhibited the expression of tyrosine kinase,;the highest inhibition of AKT phosphorylation by echinomycin was seen at the concentration of 2.0μg/L.Conclusion echinomycin can inhibit the proliferation of K562 cells,and the underlying mechanism may be related to inhibition of Bcr-Abl and reduction of protein kinase B.phosphorylated AKT.

关 键 词：棘霉素 K562 细胞周期 BCR-ABL AKT 

分 类 号：R966[医药卫生—药理学]