木犀草素对粪肠球菌毒力因子作用机制的研究  

The effects of luteolin on the virulence factor of Enterococcus faecalis

作　　者：李雪[1] 许颖[1] 赵波[1] 

机构地区：[1]佳木斯大学附属第二医院,黑龙江佳木斯154007

出　　处：《中国微生态学杂志》2016年第1期34-37,共4页Chinese Journal of Microecology

基　　金：佳木斯大学研究生科技创新项目(LM2015-064)

摘　　要：目的探讨木犀草素对粪肠球菌生物被膜的抑制效果,并进一步研究木犀草素对粪肠球菌毒力因子转录表达水平的影响。方法建立粪肠球菌的体外生物被膜模型,5组实验组分别加入浓度为0.5、1、2、4和8mg/mL的木犀草素,同步培养24h后,通过MTT检测各组的抑菌率。利用激光共聚焦显微镜观察不同浓度的木犀草素作用24h后的抑菌效果。最后选取适宜的浓度(2、4、8mg/mL)的木犀草素与粪肠球菌共同培养24h后,通过RT-PCR观察各组粪肠球菌毒力因子gelE、esp、ebpA的转录表达水平。结果 MTT和CLSM结果显示:随着浓度的增加,木犀草素对粪肠球菌生物被膜的抑制效果越来越好(P<0.05)。其中以8mg/mL组的抑菌效果最好;浓度为2、4、8mg/mL的木犀草素对其毒力因子gelE、esp、ebpA的mRNA的表达均有较好的抑制作用,并且随着药物浓度的增加木犀草素对其毒力因子的抑制效果也越来越好(P<0.05)。当药物浓度为8mg/mL时,可完全抑制gelE、esp、ebpA的mRNA的转录表达。结论木犀草素对粪肠球菌生物被膜有抑制作用,与此同时还能不同程度的抑制其毒力因子gelE、esp、ebpA的转录表达水平。Objective To investigate the inhibitory effect of luteolin on Enterococcus faecalis biofilm and further the expression of the virulence gene.Methods Enterococcus faecalis biofilm was cultured in vitro.The Enterococcus faecalis biofilm was co-cultured with 0.5,1,2,4and 8mg/mL of luteolin respectively for 24 hours.MTT assay was conducted to determine the inhibition rates of Enterococcus faecalis biofilm.The bacterial growth was observed by using CLSM.The expression levels of gelE,esp and ebpA mRNAs were observed with RT-PCR.Results The inhibitory effect of luteolin increased along with the increase of concentration（P〈0.05）.The highest inhibition was achieved at 8mg/mL of luteolin.Moreover,luteolin inhibited the mRNA expressions of gelE,esp and ebpA in a concentration dependent manner（P〈0.05）.Luteolin at 8mg/mL completely inhibited the mRNA expressions of gelE,esp and ebpA.Conclusion Luteolin can inhibit the proliferation of Enterococcus faecalis biofilm and the expression of virulence factors gelE,esp and ebpA mRNAs of Enterococcus faecalis.

关 键 词：木犀草素 毒力因子 粪肠球菌 MTT RT-PCR 

分 类 号：R285.5[医药卫生—中药学]