调肝理脾方对原发性胆汁性肝硬化小鼠调节性T细胞及肝组织病理的影响  

Effect of Tiaogan Lipi Fang(调肝理脾方) on the Regulation of T Cells and Liver Tissues in Mice With Primary Biliary Cirrhosis

作　　者：郭晓霞[1] 李良学[1] 武玉鹏[1] 贾力莉[1] 刘改萍[1] 白崇智[1] 

机构地区：[1]山西省中医院,山西省太原市并州西街46号030012

出　　处：《中医杂志》2016年第2期161-165,共5页Journal of Traditional Chinese Medicine

基　　金：山西省卫生厅科研课题计划(201301019)

摘　　要：目的探讨调肝理脾方治疗原发性胆汁性肝硬化的可能作用机制。方法 120只C57BL/6小鼠随机分为正常组、模型组、阳性组和治疗组,每组30只。除正常组外其余各组应用聚肌胞苷酸腹腔注射(5 mg/kg,2次/周,共24周)建立原发性胆汁性肝硬化动物模型,造模同时阳性组给予熊去氧胆酸胶囊0.1 g/(kg·d),治疗组给予调肝理脾方27 g/(kg·d),正常组及模型组给予等体积生理盐水,每日灌胃1次,共24周。于实验第8、16、24周时检测各组小鼠血清抗线粒体抗体M_2亚型(AMA-M2)阳性率、外周血CD4^+CD25^+Foxp3^+调节性T细胞(Treg)细胞计数、肝组织病理学检查及肝组织细胞角蛋白19(CK19)表达。结果除正常组外,第8、16、24周时各组血清AMA-M_2阳性率比较差异均无统计学意义(P>0.05)。与正常组比较,模型组小鼠第8、16、24周时外周血CD4^+CD25^+Foxp3^+Treg细胞计数明显下降(P<0.05);在第24周时,阳性组和治疗组外周血CD4^+CD25^+Foxp3^+Treg细胞计数均较模型组升高(P<0.05),且治疗组升高较阳性组明显(P<0.05)。与模型组比较,治疗组在第8、16、24周时肝组织病理分期差异有统计学意义(P<0.05)。与正常组比较,模型组第8、16、24周时CK19表达均明显升高(P<0.05);与模型组比较,治疗组第24周时CK19表达降低(P<0.05)。结论调肝理脾方能够上调原发性胆汁性肝硬化模型小鼠外周血CD4^+CD25^+Foxp3^+Treg细胞计数、降低肝组织CK19蛋白表达,从而改善胆小管损伤和肝组织纤维化。Objective To study the possible mechanism of Tiaogan Lipi Fang（ 调肝理脾方） in the treatment of primary biliary cirrhosis. Methods Totally 120 C57 BL /6 rats were randomly divided into normal group,model group,positive group and treatment group,with 30 rats in each group. Except the normal group,the rats in the other groups received intraperitoneal injection of poly I-poly（ C） 5 mg / kg,twice a week,for 24 weeks to establish the animal model of primary biliary cirrhosis. Meanwhile,the positive group was given Ursodeoxycholic Acid Capules 0. 1 g /（ kg·d）,the treatment group was given Tiaogan Lipi Fang 27g /（ kg·d）,the normal group and the model group were given equal volume of normal saline,all by gavage once a day for 24 weeks. At the 8th,16 th,and 24 th week of the experiment,the positive rate of anti mitochondrial antibody M_2 subtype（ AMA-M_2）,peripheral blood CD4^＋CD25^＋Foxp3^＋regulatory T cell（ Treg） count,and liver tissue pathological examination and expression of liver tissue and cell keratin 19（ CK19） were detected in all rats. Results There was no significant difference in the positive rate of AMA-M2 between the model group,positive group and treatment group at the 8th,16 th and 24 th week（ P〈0. 05）. Compared with the normal group,the number of CD4^＋CD25^＋Foxp3^＋Treg cells in peripheral blood in the model group was significantly decreased at 8th,16 th and 24 th week（ P〈0. 05）; At the 24 th week,the number of CD4^＋CD25^＋Foxp3^＋Treg cells in peripheral blood in the positive group and the treatment group was higher than that in the model group,but the treatment group was significantly higher than that in the positive group（ P〈0. 05）.There was significant difference in the pathological staging of liver tissues between the treatment group and the model group at the 8th,16 th and 24 th week（ P〈0. 05）. Compared with the normal group,the expression of CK19 in the model group was much higher than that in normal group at the 8th,16 th and

关 键 词：原发性胆汁性肝硬化 调肝理脾方 调节性T细胞 肝组织病理 细胞角蛋白19 

分 类 号：R285.5[医药卫生—中药学]