过敏性休克小鼠血清中类胰蛋白酶、类糜蛋白酶及PAR-2的表达  

The expression of tryptase,chymase and PAR-2 in anaphylactic shock mice

作　　者：陈洁[1,2] 曹建平[1] 崔海洋[1] 谢华[1] 

机构地区：[1]沈阳军区总医院呼吸内科,110840 [2]大连医科大学研究生院,116000

出　　处：《免疫学杂志》2016年第2期119-123,共5页Immunological Journal

基　　金：辽宁省自然科学基金(201202244);2014年辽宁省临床能力建设项目(LNCCC-A06-2014)

摘　　要：目的探讨蛋白酶激活受体2(PAR-2)在过敏性休克中的作用,为过敏性休克的治疗提供新的思路。方法昆明种雄性小鼠随机分成致敏组和对照组。致敏组小鼠于第1天腹腔注射致敏原,1周后加强注射1次,饲养3周后尾静脉注射致敏原诱发过敏性休克。对照组以同等量的PBS缓冲液代替。酶联免疫吸附法(ELISA)检测致敏组和对照组小鼠血清类胰蛋白酶及类糜蛋白酶含量;RT-PCR法检测血液中PAR-2相对表达量。结果成功建立过敏性休克小鼠模型,RT-PCR结果显示致敏后小鼠PAR-2 m RNA相对表达量为3.372±0.28,对照组为0.759±0.062,2组比较有显著性差异(P<0.01);ELISA结果显示致敏后小鼠血清类胰蛋白酶含量为72.378μg/L±8.018μg/L,正常对照组为20.265μg/L±1.953μg/L,2组比较有显著性差异(P<0.01),致敏后小鼠血清类糜蛋白酶含量(16.186μg/L±0.959μg/L)低于对照组(34.905μg/L±3.972μg/L),组间比较有显著性差异(P<0.01)。结论 PAR-2可能参与了过敏性休克的发病过程。This study performed to investigate the role of protease activated receptor 2 （PAR-2） in anaphylactic shock and provide new ideas for the treatment of anaphylactic shock. Kunming mice were randomly divided into two groups： sensitized and control groups. Mice in sensitized group were immunized by intraperitoneal injection of allergen at first day and then a booster injection was executed a week after. Anaphylactic shock was induced by tail vein injection three weeks later. Mice in control group were injected equal amount of PBS buffer. Enzyme-linked immunosorbent assay （ELISA） was used to detect the content of tryptase and chymase in mouse serum; RT-PCR was used to detect the relative expression of PAR-2 mRNA. The results indicted that the relative expression of PAR-2 mRNA in sensitized group （3.372±0.28） increased significantly as compared with the control group （0.759±0.062）（P〈0.01）. ELISA results indicated that the expression of tryptase in sensitized group （72.378 μg/ L±8.018 μg/L） increased significantly as compared with the control group （20.265 μg/L±1.953 μg/L） （P〈0.01）, while the expression of ehymase in sensitized group （16.186μg/L±0.959 μg/L） decreased significantly as compared with control group （34.905μg/L ±3.972μg/L） （P〈0.01）. Taken together, we can draw a conclusion that PAR-2 may involve in the pathogenesis of anaphylactic shock.

关 键 词：过敏性休克 蛋白酶激活受体2 类胰蛋白酶 类糜蛋白酶 

分 类 号：R593.1[医药卫生—内科学]