一种生物X射线小角散射光束线站自动换样溶液蠕动装置  

An automatic solution-sample-changing peristaltic device at biological small angle X-ray scattering beamline

作　　者：洪春霞[1] 周平[1] 李怡雯[1,2] 曾建荣[1] 边风刚[1] 王劼[1] 

机构地区：[1]中国科学院上海应用物理研究所张江园区,上海201204 [2]中国科学院大学,北京100049

出　　处：《核技术》2016年第1期6-12,共7页Nuclear Techniques

基　　金：国家重点基础研究发展计划(973计划)项目(No.2011CB911104);国家自然科学基金(No.11305242)资助~~

摘　　要：利用同步辐射X射线小角散射技术可以研究溶液中蛋白质的结构信息。但是在实验过程中,高通量的X射线易造成蛋白质的辐射损伤,发生结构变化。本文介绍了一种自动换样溶液样品蠕动装置,在实验过程中利用Hamilton的PSD/4注射泵控制样品上下运动,减小单位体积照射时间以降低X射线对蛋白质的辐射损伤。此外,通过对注射泵、准直调节台和样品/缓冲液支撑台的协调控制实现了自动换样、回样和清洗功能,提高了实验效率。在上海光源生物X射线小角散射实验站进行了实验,通过对静止模式和蠕动模式下溶菌酶的散射曲线及回旋半径的测量,表明该装置可达到很好的防辐射损伤效果,实现了预期的样品蠕动装置功能。Background： Protein structure in solution can be studied by small-angle X-ray scattering techniques at synchrotron radiation facilities. However, the structure of protein is prone to vary as the radiation damage induced by the intense X-ray beam during the experiments. Purpose： This study aims to develop an automatic solution-samplechanging peristaltic device for biological small angle X-ray scattering experiment. Methods： The injection pump PSD/4 of Hamilton is applied to eliminate the radiation damage of protein by minimizing the X-ray irradiation time of unit volume. In addition, automatic sample-changing, retrieving and cleaning was realized by the coordinated control of PSD/4, the alignment control stage and the sample/buffer control stage. Thus the experiment efficiency is improved. Results： The scattering curves and gyration radius of lysozyme under stationary collection mode and peristaltic collection mode were measured by experiments carried out at biological small angle X-ray scattering beamline of Shanghai Synchrotron Radiation Facility （SSRF）. And results show that this device can prevent radiation damage of sample efficiently. Conclusion： The designed functionalities have been achieved as expected.

关 键 词：实验物理及工业控制系统 生物X射线小角散射 控制系统工具箱 辐射损伤 

分 类 号：TP273.5[自动化与计算机技术—检测技术与自动化装置]