大鼠肩袖功能评分量表的设计及初步验证  

ESTABLISHMENT AND TESTING OF RATING SCALE OF RAT ROTATOR CUFF FUNCTION

作　　者：丁舒晨 方学伟[2] 虞荣斌[3] 刘振刚[1] 符楚迪[1] 来毅[4] 张志武[1] 卢一生[1] 

机构地区：[1]解放军第117医院骨科中心,杭州310001 [2]西安交通大学机械工程学院 [3]宁波市医疗中心李惠利医院防保科 [4]西安邮电大学通信与信息工程学院

出　　处：《中国修复重建外科杂志》2016年第1期54-59,共6页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的通过测量冈下肌功能不同程度丧失大鼠的步态参数,设计大鼠肩袖功能评分量表,并进行初步动物模型验证,为客观评价大鼠肩袖功能提供工具。方法取成年雄性SD大鼠60只进行实验,体质量（281.21±20.12）g。其中48只制备冈下肌功能不同程度丧失大鼠模型,进行量表设计。将大鼠随机分为断裂组、肉毒素组、假手术组及正常对照组（n=12）。肉毒素组于冈下肌肌腹内注射A型肉毒素（6 U/kg）,断裂组微创暴露大鼠冈下肌肌腱并切断,假手术组仅暴露肌腱,正常对照组不作处理。7 d后采集4组大鼠自由行走时双侧前肢单位时间着地次数、支撑时相比、触地压力、摆动速度、触地速度以及双侧肢体前后脚间距离,计算各步态参数干预侧/对照侧比值,纳入断裂组、肉毒素组分别与假手术组、正常对照组间差异有统计学意义的步态参数作为量表评分项目,并根据步态参数测量结果赋值1~5分。另取12只大鼠建立慢性肩袖损伤模型,于术后8周内每周参照设计的大鼠肩袖功能评分量表进行评价。结果断裂组各步态参数均显著小于肉毒素组,肉毒素组显著小于假手术组及正常对照组,比较差异均有统计学意义（P〈0.05）;假手术组和正常对照组比较,差异均无统计学意义（P〉0.05）。将各步态参数均作为量表评价项目,各项评分1~5分,总分为30分,最低分为6分,分别反映大鼠冈下肌功能完全正常（肩袖功能完全正常）和完全丧失（肩袖功能严重丧失）。验证组大鼠第1~8周肩袖功能评分分别为（27.00±1.86）、（23.75±2.83）、（21.33±1.92）、（18.17±2.37）、（13.17±1.64）、（11.67±2.50）、（8.17±1.27）、（6.50±0.67）分。结论根据冈下肌功能不同程度丧失大鼠模型设计了大鼠肩袖功能评分量表,并通过慢性大鼠肩袖损伤模型初步验证,该量表能在一定程度上反映大鼠肩袖损伤程度及肩袖�Objective To provides an objective tool to evaluate rotator cuff function in rats. Methods Sixty adult male Sprague Dawley rats, weighing（281.21± 20.12） g, were involved in this experiment. Botulinum toxin A（6 U/kg） was injected into the infraspinatus of 12 rats in Botox group. Infraspinatus tendons of 12 rats in tear group were cut by microinvasive method. Infraspinatus tendons of 12 rats in sham-operation group were exposed but not cut. No any treatment was performed on the 12 rats in normal control group. One shoulder joint of each rat was selected randomly as treatment side. After 7 days, cadence, rate of stand phase, support pressure, swing speed, stand speed, and stride length of rats in those 4 groups were measured. Treatment side and contralateral side ratio of each gait parameter was caculated. If parameters of Botox group and tear group both showed significant differences compared with those of normal control group and sham-operation group, the gait parameter was brought into rating scale as an item. Each item was assigned from 1 point to 5 points according to gait parameter. The chronic rotator cuff injury model was established in 12 rats of verification group and rating scale was used to evaluate rotator cuff function each week for 8 weeks after surgery. Results All gait parameters of tear group were significantly less than those of Botox group（P〈0.05）, and Botox group was significantly less than sham-operation group and normal control group（P〈0.05）, but no significant difference was found between sham-operation group and normal control group（P〉0.05）. All gait parameters were brought into rating scale as items. All items were rated from 1 point to 5 points, so the total score was 30 points and the lowest score was 6 points. Thirty points meant normal function of infraspinatus（rotator cuff function was normal）, and 6 points meant complete loss of infraspinatus function（rotator cuff function was severely damaged）. Rotator cuff functional scores of rats in verification

关 键 词：肩袖功能 评分量表 步态分析 大鼠模型 

分 类 号：R686[医药卫生—骨科学]