新疆维吾尔族、汉族慢性乙型肝炎患者HLA-DQA1基因多态性研究  

Study on the polymorphism of HLA-DQA1 gene in hepatitis B patients of Uygur and Han nationality lived in Xinjiang region

作　　者：杨丽[1] 李真 徐菲莉[1] 

机构地区：[1]新疆医科大学附属中医医院临床检验中心,乌鲁木齐830000 [2]新疆维吾尔自治区第六人民医院检验科,乌鲁木齐830000

出　　处：《重庆医学》2016年第1期1-4,共4页Chongqing medicine

基　　金：新疆维吾尔自治区自然科学基金资助项目(2013211A117)

摘　　要：目的探讨人类白细胞抗原-DQA1(HLA-DQA1)基因多态性与新疆维吾尔族(以下简称维族)、汉族慢性乙型肝炎患者遗传易感性的关系,找寻新疆维族与汉族慢性乙型肝炎患者乙型肝炎病毒(HBV)感染的易感基因和拮抗基因及维、汉族之间HLA-DQA1基因型差异。方法选择乙型肝炎组患者182例(维族102例,汉族80例),健康对照组163例(维族85例,汉族78例);根据乙型肝炎患者血清HBV DNA病毒载量的不同分为高复制组和低复制组;根据血清丙氨酸氨基转移酶(ALT)水平分为ALT正常组和ALT异常组。PCR-序列特异性引物(PCR-SSP)法检测HLA-DQA1*0102、-DQA1*0104、-DQA1*0201、-DQA1*0301、-DQA1*0302、-DQA1*0501等6个等位基因的频率分布;采用酶促反应法检测ALT;PCR检测HBV DNA。结果维族乙型肝炎患者组、ALT异常组、HBV DNA高复制组与健康对照组比较-DQA1*0301、-DQA1*0501基因频率差异有统计学意义(P<0.05)。汉族乙型肝炎患者ALT异常组、HBV DNA高复制组与健康对照组基因频率比较-DQA1*0102、-DQA1*0201、-DQA1*0301差异有统计学意义(P<0.05),HBV DNA高复制组与低复制组等位基因-DQA1*0102比较差异有统计学意义(P<0.05);HBV DNA低复制组、ALT正常组与健康对照组中HLA-DQA1*0201位点基因比较,差异有统计学意义(P<0.05)。维族与汉族慢性乙型肝炎患者HLA-DQA1等位基因频率比较,-DQA1*0102、-DQA1*0301基因频率差异有统计学意义(P<0.05)。维族与汉族健康对照HLA-DQA1等位基因频率比较,-DQA1*0201、-DQA1*0501基因频率差异有统计学意义(P<0.05)。结论维族人群中HLA-DQA1*0501为HBV感染拮抗基因,-DQA1*0301为易感基因。汉族人群中HLADQA1*0102、-DQA1*0301、-DQA1*0302为HBV感染易感基因,-DQA1*0201为拮抗基因。Objective To investigate the relation between the human leucocytes antigen-DQA1 （HLA-DQA1） alleles polymorphism and the Uygur Han nationality hepatitis B patients in Xinjlang and the genetic susceptibility healthy controls, which is provide some important clues to seek the susceptible genes and disease-resistant genes of HBV infection for Uygur and Han nationality hepatitis B patients. Methods HLA-DQA1 alleles of 182 cases of the Hepatitis B patients and 163 people were compared with HBV DNA and ALT level. HLA：DQA1 ＊ 0102,-DQA1 ＊ 0104,-DQA1 ＊ 0201 ,-DQA1 ＊ 0301 ,-DQA1 ＊ 0302,-DQA1 ＊ 0501 genes frequency are detected with PCR-SSP. Results Compared the Uygur hepatitis B patients ALT abnormal and HBV DNA high copy quantity group with healthy controls group in alleler s frequency analysis found that HLA-DQA1 ＊ 0301,-DQA1 ＊ 050t genes had statistic significance（P〈0.05）. Compared the Han nationality hepatitis B patients ALT abnormal and HBV DNA high copy quantity group with healthy controls group in allele＇s frequency analysis, It was found that HLA-DQA1 ＊ 0102,-DQA1 ＊ 0201 ,-DQA1 0301 genes difference had statistic significance（P〈0.05）. HLA-DQA1 ＊ 0102 had the statistic significance between high and low copy quantity groups（P〈0.05）. Compared the Han nationality hepatitis B patients ALT normal and low copy quantity group with healthy controls group in allelers frequency analysis, It was found that HLA-DQA1 ＊ 0201 genes had statistic significance（P〈 0.05）. HLA-DQA1 ＊ 0102,-DQA1 ＊ 0301 had statistic significance between the Han and Uygur nationality for HBV patients（P〈 0.05） ; HLA-DQA1 ＊ 0201 ,-DQA1 ＊ 0501 had statistic significance between the Han and Uygur nationality for healthy people（P〈 0.05）. Conclusion HLA-DQA1 ＊ 0501 is the protected gene of Uygur hepatitis B patients ;-DQA1 ＊ 0301 is the susceptibility gene. The Han nationality hepatitis B patients group HLA-DQA1 ＊ 0102,-DQA1 ＊ 0301,-DQA1 ＊ 0302 is the susceptibility genes and

关 键 词：人白细胞抗原 DQA1 维吾尔族 汉族 肝炎 乙型 慢性 

分 类 号：R512.62[医药卫生—内科学] R446.1[医药卫生—临床医学]