腹膜透析液对腹膜间皮细胞TLR2和TLR4表达的影响  

Effect of peritoneal dialysis fluids on the expression of TLR2 and TLR4 on peritoneal mesothelial cells

作　　者：伍军[1] 何敏[1] 张健[1] 何文飞[1] 程兵[1] 

机构地区：[1]汕头大学医学院附属粤北医院肾内科,广东韶关512026

出　　处：《重庆医学》2016年第2期156-158,163,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81200555)

摘　　要：目的观察不同浓度葡萄糖（dextrose）腹膜透析液、艾考糊精（ieodextrin）腹膜透析液对人腹膜问皮细胞Toll样受体2（TLR2）和TLR4表达的影响。方法体外培养人腹膜闯皮细胞株第5～10代（HMrSV5，DMEM／F：Z培养基含10％胎牛血清）用于实验研究。MTT检测细胞活力，实验分为5组：阴性对照组、1．50％dextrose组、2．50％dextrose组、4．25％dextrose组、7．5％icodextrin组，应用蛋白免疫印迹法（westernblot）检测TLR2和TLR4的表达水平。结果不同浓度dextrose腹膜透析液作用24h对腹膜间皮细胞TLR2和TLR4的表达无明显影响。作用48h，1．50％dextrose组、2．50％dextrose组、4．25％dextrose组TLR2分别降低（5．5±2．8）％、（31．4±7．5）％、（54．9±1.9）％，TLR4分别降低（32．9±17．6）％、（47.7±13．5）％、（66．4±13．5）％；作用72h，1．50％dextrose组、2．50％dextrose组、4．25％dextrose组TLR2表达分别降低（29．4±14．7）％、（38．9±9．9）％、（63．5±16．5）％，TLR4表达分别降低（59．5±16．8）％、（63．1±9．5）％、（79．2±14．0）％；7．5％icodextrin组TLR2和TLR4表达与阴性对照组比较差异无统计学意义（P〉0．05）。结论dextrose透析液下调HMrSV5 TLR2和TLR4的表达；icodextrin透析液对TLR2和TLR4的表达无显著影响。Objective To investigate the effect of glucose-based peritoneal dialysis fluids and icodextrin-based peritoneal dial- ysis fluids on the expression of TLR2 and TLR4 on huamn peritoneal mesothelial cells. Methods Human peritoneal mesothetial cell line 5-10 generations（HMrSVS） was cultured in DMEM/F12 medium supplemented with 10% （v/v） fetal calf serum （FCS）. Cell viability and cell proliferation were assessed using MTT method. The experiment were divided into 5 different groups; group A （control group）, 1.5 %dextrose group,2.5 % dextrose group, 4.25 % dextrose group and 7.5 % Lcodextrin group. Icodextrin group （aikau dextrin） ,TLR2 and TLR4 expression were detected by Western blot. Results Treatment with different concentrations of glucose-based peritoneal dialysis fluids for 24 h did not affect the expression of TLR2 and TLR4 protein. In addition,after stimulation for 48 h, 1. 5 % dextrose, 2. 5 % dextrose. 4. 25 % dextrose decreased TLR2 expression by （ 5. 5 ± 2.8）%, （31.4±7.5）%, （54.9± 1.9）% respectively, TLR4 expression by （32.9±17.6）%, （47.7 ± 13.5）%, （66.4± 13.5）% respectively. Stimulation for 72 h,decreased TLR2 expression by （29.4±14.7）% ,（38.9±9.9）% ,（63.5±16.5）% respectively,TLR4 expression by（59.5±16.8） %, （63.1 ±9.5）%, （79.2 ± 14.0）% respectively. There was no significant change in TLR2 and TLR4 protein expression on 7.5% icodextrin group. Conclusion Glucose-based peritoneal dialysis fluids, but not icodextrin-based peritoneal dialysis fluids downregulates expression of TLR2 and TLR4 by HMrSV5.

关 键 词：腹膜透析 人腹膜间皮细胞 TOLL样受体2 TOLL样受体4 

分 类 号：R459.5[医药卫生—治疗学]