CD14基因在大白猪F18大肠杆菌病耐受型和敏感型个体间的差异表达分析  

Analysis of CD14 Gene Expression in Sensitive and Resistant Yorkshire Pigs to E coli F18

作　　者：夏日炜 宗秋芳 庄亚润 刘颖[1] 吴圣龙[1,2] 包文斌[1,2] 

机构地区：[1]扬州大学动物科学与技术学院,江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [2]江苏省种猪繁育和健康养殖工程技术研究中心,江苏扬州225009

出　　处：《中国畜牧杂志》2016年第1期13-16,共4页Chinese Journal of Animal Science

基　　金：江苏省科技支撑计划(BE2015329,BE2014357,BE2014310);常州市科技支撑计划(CE2013011)

摘　　要：本研究旨在分析CD14基因在大白猪F18大肠杆菌耐受型和敏感型个体间的mRNA表达差异,以探讨CD14基因在断奶仔猪抗F18大肠杆菌中发挥的潜在作用。挑选大白猪耐受型和敏感型个体各4头,通过利用Real-time PCR方法比较分析CD14基因在个体各组织间的表达规律。结果表明:CD14基因在大白猪耐受型和敏感型个体各组织间的表达趋势有着高度的一致性,其中CD14基因在肝中呈现高水平表达,在肺、肾和淋巴结中呈现中度表达,而在其他组织中的表达水平都非常低,CD14基因在耐受型个体各组织间的表达水平几乎都高于敏感组,其中CD14基因表达量在空肠组织中差异达到极显著水平(P<0.01)。由此推测,CD14基因对E.coli F18抗性的调节可能不是通过直接作为F18大肠杆菌的受体来实现,而是通过参与并调节机体的免疫反应来实现的,并且CD14基因的上调可能与F18大肠杆菌的抗性存在一定的联系。This study aimed at investigating the differential mRNA expression of CD14 gene between sensitive and resistant Yorkshire pigs to E coli F18, and exploring the potential role of CD14 gene in E. coli F18 resistance of weanling pigs. The expression level of CD14 gene in each tissue, which was obtained from the four Yorkshire pigs which come from sensitive and resistant groups separately, was detected by the method of Real-time PCR. The result showed that the tendency of expression of CD14 gene between sensitive and resistant Yorkshire pigs was very similar. The expression level of CD14 gene was the highest in liver, and moderate in lung, kidney and lymph. However, the level was very low in the other tissues. The expression of CD14 gene in resistant group was higher than it in sensitive group nearly, and the expression of CD14 gene had significant difference among jejunum tissue （P〈0.01）. It could be speculated that CD14 gene affecting the resistance to E. coli F18 by regulating the immune reaction of organism instead of being the receptor of E. coil F18. In addition, the higher expression of CD14 gene can have some impact on the resistance of E. coli FI8.

关 键 词：猪 CD14基因 F18大肠杆菌 mRNA表达 

分 类 号：S828.2[农业科学—畜牧学]