从多潜能干细胞诱导成神经前体细胞及其分化探讨  

Induction and differentiation of neural precursor cells from induced pluripotent stem cells

作　　者：武赟[1,2] 周舒雅[3] 徐群渊[2] 

机构地区：[1]山西中医学院基础医学院解剖学教研室,山西晋中030619 [2]首都医科大学神经生物学系,北京100069 [3]中国科学院动物研究所,北京100101

出　　处：《解剖学报》2016年第1期18-22,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金(81070977;81200931;81271388)

摘　　要：目的探讨诱导多潜能干细胞在体外分化为神经前体细胞。方法采用N2B27作为选择培养基,多步法贴壁培养把诱导多潜能干细胞诱导为神经前体细胞。结果免疫荧光染色发现,该细胞神经干细胞的标记物巢蛋白阳性,有极个别的β-微管蛋白Ⅲ(β-Tub-Ⅲ)阳性细胞。在分化培养基内培养7d后,β-Tub-Ⅲ阳性细胞增多。结论本实验初步表明,诱导多潜能干细胞在体外可以被诱导为有一定神经特征的神经前体细胞,该细胞有望用于细胞移植治疗缺血性脑损伤。Objective Induced pluripotent stem cells （iPSCs） differentiate into neural precursor cells （NPCs） in vitro. Methods The N2B27 was used as medium in a muhistep adherent culture to induce the iPSCs into the NPCs. Results Immunofluorescenee staining showed that the Nestin, the neural stem cell marker, was positive in the NPCs. There were a few β-tubulin Ⅲ （ β-Tub-Ⅲ） positive NPCs. After 7 days culture in the differentiation culture medium, β-Tub-]ll positive NPCs increased. Conclusion This study confirms that the iPSCs can be induced into the NPCs in vitro, which may be used in the treatment of ischemie brain injury.

关 键 词：诱导多潜能干细胞 神经前体细胞 分化 细胞培养 免疫荧光 小鼠 

分 类 号：Q189[生物学—神经生物学]