Bmi-1在不同发育阶段食管上皮的表达及其意义  

Expression of Bmi-1 in different developmental stage of esophageal epithelium tissues

作　　者：慕晓玲[1] 周文斌[2] 刘洋[2] 史苏云 陈小雪[2] 裴学莲[2] 李和[1] 

机构地区：[1]华中科技大学同济医学院基础医学院组织学胚胎学教研室,武汉430030 [2]石河子大学医学院组织学胚胎学教研室,新疆石河子832002

出　　处：《解剖学报》2016年第1期95-101,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金(31460310);新疆生产建设兵团科技支疆计划(2013AB024);石河子大学国家级大学生创新创业训练计划(201510759058)

摘　　要：目的观察Pc G家族蛋白Bmi-1在食管上皮组织中的表达情况,探讨Bmi-1在食管上皮组织发育过程中的表达规律及意义。方法采用免疫组织化学法检测120例小鼠胚胎、20例成年小鼠、15例人体胚胎、18例正常成年人食管上皮组织及17例食管癌组织中Bmi-1的表达,并利用图像分析软件对Bmi-1的表达强度进行定量分析,运用统计学软件对各组数据进行统计学分析。结果 Bmi-1在各组食管上皮组织及食管癌组织中均存在表达,但各组表达强度不同,差异有统计学意义(P<0.05)。Bmi-1在小鼠与人体胚胎时期食管上皮组织中的表达量均高于生后食管上皮组织。Bmi-1在人体胚胎、正常成年人食管上皮及食管癌组织中表达强度不同,差异有统计学意义(P<0.01),并且在食管癌组织中呈强阳性表达。结论 Bmi-1在不同发育阶段食管上皮组织的表达强度不同,与食管上皮组织的发育密切相关。Objective To observe the Bmi-1 protein expression in esophageal epithelial tissues,and to explore the relationship between the expression pattern of Bmi-1 and the development of esophageal epithelial tissues. Methods Immunohistochemistry was used to measure the expression of Bmi-1 in esophageal epithelial tissues of 120 mice in different development time, 15 human embryos, 18 normal human adults and 17 human esophageal squamous cell carcinoma （ESCC） samples. The level of Bmi-1 protein expression in these samples was analyzed by an image analysis software, and the statistical analyses were applied to test the relationship between the expression of Bmi-1 and each group. Results Bmi- 1 was found to express in every time of development, but the differences were present in the protein expression level （P 〈 O. 05）. Expression of Bmi-1 protein was higher at embryo stage than that after birth in both mice and human. Compared with esophageal tissue of normal human adult, Bmi-1 overexpressed both in human esophageal squamous cell carcinoma and human embryo（P 〈 0. 01 ）. Conclusion The expression of Bmi-1 closely is related with the development of esophageal epithelium. Furthermore, there is a potential significance to detection of the Bmi-1 expression for the research in the developmental biology of esophageal epithelium and the cytobiology of esophageal cancer.

关 键 词：BMI-1 食管上皮组织 发育 免疫组织化学 小鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]