非肌性肌球蛋白Ⅱ-B调节斑马鱼咽弓的发育  

Nonmuscle myosin Ⅱ-B regulates the development of pharyngeal arches in zebrafish

作　　者：王鸿奎[1] 王学谦[1] 王新[1] 袁玮[1] 刘东[1] 

机构地区：[1]南通大学江苏省神经再生重点实验室,江苏南通226001

出　　处：《解剖学报》2016年第1期113-116,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金(31201083;81570447;31400918)

摘　　要：目的探讨非肌性肌球蛋白Ⅱ(NMⅡ)家族基因在斑马鱼胚胎期咽弓的表达及其对咽弓发育的影响。方法通过斑马鱼胚胎整体原位杂交及冷冻切片技术(n=10),观察NMⅡ家族基因在斑马鱼胚胎期咽弓的表达情况。利用软骨的阿辛蓝染色方法,将野生型对照组(n=10)和blebbistatin处理组(每个浓度n=10)的斑马鱼胚胎进行比较,分析NMⅡ对斑马鱼胚胎期咽弓发育和形成的影响。结果受精后72 h(72hpf),NMⅡ-B在斑马鱼发育着的咽弓部位有特异性表达;120 hpf,NMⅡ-B在咽弓表达增加。Blebbistatin特异性抑制NMⅡ-B功能后,斑马鱼咽弓发生明显发育缺陷,部分软骨细胞出现显著形态改变;抑制咽弓形成的作用与blebbistatin浓度呈现剂量依赖效应。结论 NMⅡ-B通过影响咽弓软骨细胞从而调节斑马鱼胚胎期咽弓的发育。Objective To analyze the expression of nonmuscle myosin Ⅱ （NM Ⅱ ） in pharyngeal arches and the function of NM Ⅱ on the development of pharyngeal arches in zebrafish embryos. Methods The expression of NM Ⅱ -B in pharyngeal arches in zehrafish embryos was observed by whole-mount in situ hybridization and cryostat section （ n = 10）. The function of NM Ⅱ-B on the development of pharyngeal arches in zebrafish embryos was analyzed following the comparison of wild type （n = 10） and blehbistatin-treated （n = 10 per concentration） zebrafish embryos by cartilage Alcian blue staining. Results The expression of NM Ⅱ-B in pharyngeal arches in zebrafish was detected at 72 hours post- fertilization （hpf）; the expression of NM Ⅱ-B in pharyngeal arches increased at 120 hpf. The pharyngeal arches in zebrafish appeared obvious developmental defects, and a few of chondrocytes showed significant morphological changes after Blebbistatin inhibition of NM Ⅱ -B functions, which was at a dose-dependent manner. Conclusion NM Ⅱ-B regulates the development of pharyngeal arches through affecting the chondrocytes of pharyngeal arches in zebrafish embryos.

关 键 词：非肌性肌球蛋白Ⅱ-B 咽弓 发育 原位杂交 阿辛蓝染色 斑马鱼 

分 类 号：R394.1[医药卫生—医学遗传学]