补肾益气方联合有氧运动对衰老大鼠骨骼肌线粒体生物合成相关信号分子表达的影响  

The influence of Bushen Yiqi Decoction and aerobic exercise on the expression of mitochondria biosynthesis related signaling molecules in the aging rats' skeletal muscle

作　　者：徐划萍[1] 金国琴[2] 韩志芬[2] 

机构地区：[1]上海中医药大学体育部,上海201203 [2]上海中医药大学基础医学院,上海201203

出　　处：《上海中医药杂志》2016年第1期74-78,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81473583);上海市教委资助项目(2012JW29;085ZY1204)

摘　　要：目的观察补肾益气方及有氧运动对D-半乳糖所致衰老大鼠骨骼肌线粒体生物合成相关信号分子丝裂原活化蛋白激酶(p38MAPK)、钙调蛋白激酶Ⅱ(Ca MKⅡ)、过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)、心肌细胞增强因子2C(MEF2C)及细胞色素氧化酶(COXⅣ)表达的影响。方法将40只雄性SD大鼠随机分为5组,分别为正常组、模型组、中药组、运动+中药组、美雄酮组。除正常组外,其余各组每天颈背部皮下注射10%的D-半乳糖(100μg/g)溶液,制作"拟衰老"大鼠模型。模型大鼠分别经补肾益气方药、有氧跑台运动、美雄酮干预10周后,采用ELISA法测定大鼠血清β-半乳糖苷酶(β-Galase)含量,采用甲基百里香酚蓝法(MTB)检测大鼠腓肠肌线粒体钙含量,采用Western blotting法检测大鼠腓肠肌线粒体生物合成信号通路分子p38MAPK、Ca MKⅡ、PGC-1α、MEF2C及COXⅣ蛋白表达变化。结果与正常组比较,模型组大鼠衰老生物学标志物β-Galase、线粒体钙含量显著增高,Ca MKⅡ、MEF2C、PCG-1α蛋白表达明显下降,而P38MAPK蛋白表达显著升高(P<0.05,P<0.01)。与模型组比较,中药组可显著降低血清β-GALase蛋白含量、线粒体钙含量(P<0.01);运动+中药组可显著降低线粒体钙含量,上调Ca MKⅡ、PGC-1α、MEF2C及COXⅣ蛋白表达;美雄酮组显著降低线粒体钙含量,上调PCG-1α、COXIV蛋白表达(P<0.05,P<0.01)。结论衰老大鼠骨骼肌功能减退可能与线粒体生物合成相关信号转导通路分子Ca MKⅡ、p38 MAPK、PCG-1α、MEF2C及能量代谢相关酶蛋白基因(COXIV)表达异常变化有关;补肾益气方联合有氧运动可一定程度改善上述指标的异常变化,延缓骨骼肌衰老。Objective To observe the influence of Bushen Yiqi Decoction and aerobic exercise on the expression of mitochondria biosynthesis related signaling molecules p38 MAPK,Ca MKⅡ,PGC-1α,MEF2 C and COXⅣ in D- galactose-induced aging rats’ skeletal muscle. Methods 40 male SD rats were randomly divided into five groups： normal group,model group,traditional Chinese medicine group,exercise and traditional Chinese medicine group and methandienone group. The rats were received a subcutaneous injection of 10% D-galactose solution（ 100 mg·kg- 1,once a day） for 10 weeks to make the aging model except normal group rats. Bushen Yiqi Decoction,aerobic exercise and methandienone were used to the corresponding experimental groups to intervene for 10 weeks. β-Galase of blood serum were tested by ELISA; Calcium content of gastrocnemius mitochondria were tested by Methyl thyme phenol blue（ MTB）; The expression of mitochondrial biosynthesis related signal transduction molecules（ p38 MAPK,Ca MKⅡ,PGC-1α,MEF2 C and COXⅣ） were tested by Western blot respectively.Results Compared with normal group,model group rats’ aging biology markers β-Galase,calcium content in mitochondria increased significantly. The protein expression（ protein） of model rats’ Ca MKⅡ,MEF2 C,PCG-1 alpha decreased obviously,and the protein expression of P38 MAPK increased significantly. The Bushen Yiqi Decoction combined with aerobic exercise could obviously decrease calcium content,and increase the protein expression of Ca MK Ⅱ,PGC-1α,MEF2 C and COXⅣ of the model aging rats. The methandienone could obviously increase the protein expression of PCG-1 α and COXIV of the model aging rats,decrease calcium content obviously. Conclusion The decline of aging model rats’ skeletal muscle function may related to expression changes of mitochondria biosynthesis related signal transduction pathways molecular Ca MK Ⅱ,p38 MAPK,PCG-1 alpha,MEF2 C and energy metabolism related genes（ COXIV）. The Bushen Yiqi Decoction combined with aerobic

关 键 词：补肾益气方 有氧运动 衰老大鼠 骨骼肌 线粒体生物合成 

分 类 号：R285.5[医药卫生—中药学]