山竹果皮中总氧杂蒽酮对人鼻咽癌CNE2细胞增殖及凋亡的影响  

Effects of the Xanthones from Pericarps of Mangosteen on Proliferation and Apoptosis of Human Nasopharyngeal Carcinom Cell Line CNE2

作　　者：赖燕燕[1] 黄应雯 黄丹玚 伍兰岚 罗彬尤 李晓龙[3] 

机构地区：[1]广西卫生职业技术学院,南宁530021 [2]广西中医药大学第一附属医院,南宁530023 [3]广西医科大学基础医学院,南宁530021

出　　处：《医药导报》2016年第1期20-24,共5页Herald of Medicine

基　　金：广西医科大学青年科学基金项目(GXMUYSF09)实验室开放课题(02610212063)

摘　　要：目的探讨山竹果皮中总氧杂蒽酮提取纯化物对人鼻咽癌CNE2细胞增殖及凋亡的影响及其作用机制。方法将人鼻咽癌CNE2细胞随机分成阴性对照组和不同浓度山竹果皮中总氧杂蒽酮提取纯化物组。阴性对照组不加药物,正常培养;总氧杂蒽酮提取纯化物组分别以200,400,600,800μmol·L-1总氧杂蒽酮作用24,48,72 h。采用噻唑蓝(MTT)法检测不同浓度总氧杂蒽酮提取纯化物对CNE2细胞增殖的影响,Annexin-V/PI双重染色、碘化丙啶单染进行流式细胞术检测总氧杂蒽酮提取纯化物对CNE2细胞周期和凋亡的影响。Caspase-3试剂盒检测总氧杂蒽酮提取纯化物对CNE2细胞Caspase-3酶活化的影响。结果总氧杂蒽酮提取纯化物随着浓度增加,可显著抑制人鼻咽癌CNE2细胞的增殖活性,浓度为371.536 7μmol·L-1时可诱导人鼻咽癌CNE2细胞出现明显早期凋亡,并且随着药物作用时间的增加(24,48,72 h),凋亡早期癌细胞的比例显著上升(分别为0.03%,10.54%,26.47%)(P<0.05);总氧杂蒽酮提取纯化物使CNE2细胞G1期细胞比例大幅升高,同时S期细胞比例明显下降;对Caspase-3具有激活作用,Caspase-3酶的活性与药物浓度成正比。结论山竹果皮中总氧杂蒽酮提取纯化物对人鼻咽癌CNE2细胞增殖有显著抑制作用和促凋亡作用,其作用机制可能与抑制鼻咽癌细胞增殖活性、抑制细胞进入S期和激活Caspase-3有关。Objective To study the effects and mechanism of the xanthones from pericarps of mangosteen on human nasopharyngeal carcinoma cell line CNE2. Methods Human nasopharyngeal carcinoma CNE2 cells were randomly divided into negative control group and different concentration of the xanthones from pericarps of mangostees. The negative control was normally cultured without drugs; The xanthones from pericarps of mangosteen groups were respectively treated by 200,400,600,800 μmol·L-1xanthones 24,48,72 hours. MTT assay,Annexin-V / PI double staining,and PI single staining were employed to investigate the action of the xanthones from pericarps of mangosteen on the cell cycle and apoptosis of CNE2 cells.And Caspase-3kit was applied to investigate the activity of Caspase-3. Results The xanthones from pericarps of mangosteen significantly inhibited the proliferation activity of human nasopharyngeal carcinoma cell line CNE2 in a dose-dependent manner,especially for which at 371.536 7 μmol·L-1induced early apoptosis of CNE2 cells,and with the time passed by（ 24,48,72 h）,the ratio of early apoptotic cancer cell significantly elevated（ 0. 03%,10. 54%,26. 47%）（ P 〈0. 05）; Cell-cycle analysis confirmed that the xanthones from pericarps of mangosteen induced the G1-phase cell cycle arrest and blocked cells entering the S-phase.The extracts activated Caspase-3 dose dependently. Conclusion The xanthones from pericarps of mangosteen remarkablly suppress proliferation of human nasopharyngeal carcinoma cell line CNE2 and promote their apoptosis,which may be involved in inhibition cells entering the S-phase and activating Caspase-3.

关 键 词：总氧杂蒽酮 果皮 山竹 癌 鼻咽 人 CNE2细胞 

分 类 号：R282.71[医药卫生—中药学] R739.63[医药卫生—中医学]