刺萼悬钩子枝总黄酮的超声提取工艺  

Ultrasound Extraction Technology of Total Flavonoids from Rubus alexeterius Focke Branches

作　　者：李蕾[1,2,3] 孙美利 王和宇[1,2,3] 张晶[1] 徐暾海[2,3,4] 刘铜华[2,3] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]北京中医药大学中医养生学北京市重点实验室,北京100029 [3]北京中医药大学中医养生学教育部重点实验室,北京100029 [4]北京中医药大学中药学院,北京100029

出　　处：《医药导报》2016年第1期79-82,共4页Herald of Medicine

基　　金：国际科技合作与交流项目(2012DFG31550);北京中医药大学创新团队项目(2011-CXTD-19)

摘　　要：目的优化刺萼悬钩子枝中总黄酮超声提取工艺。方法采用分光光度法对总黄酮含量进行测定,考察乙醇浓度、料液比、超声时间和超声次数对总黄酮提取率的影响,并通过正交实验设计优化提取工艺。结果超声提取的最优工艺条件为:乙醇浓度50%,料液比1∶40,超声时间60 min,超声2次,总黄酮提取率38.951 mg·g-1。结论根据正交工艺及节能观念确定的最优工艺条件提取刺萼悬钩子枝中总黄酮的工艺条件合理。Objective To optimize the ultrasonic extraction process of total flavonoids from Rubus alexeterius Focke.Methods The colorimetric analysis was taken to detect the total flavonoid in terms of ethanol fraction,solid-liquid ratio,extraction duration and extraction times,and the orthogonal experimental design was used to optimize the extraction process.Results The optimum conditions for ultrasound extraction included taking ethanol volume fraction of 50%,solid-liquid ratio 1∶40,ultrasonic for 60 min twicely,and obtaining 38. 951 mg · g^-1of the total flavonoid. Conclusion The process of total flavonoids extracted on the base of the orthogonal technology and energy-saving concepts from Rubus alexeterius Focke stems are reasonable.

关 键 词：刺萼悬钩子 总黄酮 提取工艺 超声 正交实验 

分 类 号：R282.71[医药卫生—中药学] TQ460.6[医药卫生—中医学]