我国辣椒种质资源eIF4E基因多态性分析  

Analysis of Polymorphism of eIF4E in Pepper Germplasm Resources( Capsicum spp. ) of China

作　　者：王学瑛[1] 张正海[1] 王海平[1] 顾晓振 李锡香[1] 张宝玺[1] 王立浩[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《植物遗传资源学报》2016年第1期120-125,共6页Journal of Plant Genetic Resources

基　　金：公益研究所基金(ICS;CAAS)(1610032011011);国家"863"项目(2012AA100103002);中国农业科学院科技创新工程(CAAS-ASTIPIVFCAAS);创新工程农业部大宗蔬菜技术产业体系(CARS-25);农业部园艺作物生物学与种质创制重点实验室项目

摘　　要：利用国家蔬菜种质资源库的1904份辣椒资源材料,采用测序技术获得eIF4E(eukaryotic translation initiation 4E)基因exon1序列,研究e IF4E基因多样性及我国辣椒种质资源群体多样性。结果表明:在1904份材料中共发现17个单倍型,14个有义多态性位点,其中9个为新的位点,位点大多集中在eIF4E蛋白表面环上;8个地理群体的平均单倍型多样性(Hd)和平均核苷酸多样性(Pi)分别为0.519和0.00210;群体间分化指数(Fst值)及基因流(Nm)表明不同群体间表现差异的分化程度;AMOVA分析表明总变异主要来源于群体内个体间的变异(97.23%),只有2.77%变异发生在群体间。本研究将有助于了解我国辣椒eIF4E基因多样性,为抗PVY育种提供更多抗源材料。Exon 1 of eIF4E gene（ eukaryotic translation initiation 4E） of 1904 pepper accessions from National Vegetable Germplasm Bank in China was sequenced and researched on the diversity. The results showed： 17 haplotypes were found among the 1904 accessions and 9 new sites in the 14 sense polymorphic loci which mostly concentrated on the e IF4 E protein surface ring; the average haplotype diversity（ Hd） and average nucleotide diversity（ Pi） of8 geographic populations were 0. 519 and 0. 00210; differentiation index（ Fst value） and gene flow（ Nm） between populations showed that it performed different degree of differentiation among groups; AMOVA analysis showed that the total variation came mainly from individuals within populations（ 97. 23%）,only 2. 77% of the variation occurred between populations. This research is helpful to understand the diversity of the eIF4E gene,providing more resistant resources for pepper PVY resistance breeding.

关 键 词：辣椒 种质资源 真核转录因子(eIF4E) 多态性 

分 类 号：S641.3[农业科学—蔬菜学]