针刺对营养型肥胖大鼠慢性炎症状态的调节作用  

The regulatory effect of acupuncture on chronic inflammation in rat model of nutritional obesity

作　　者：夏猛[1] 蒋凌风[1] 陈丹丹[1] 梁媚[1] 黄鸿腾 张昭阳[1] 刘佳[1] 唐红珍[1] 

机构地区：[1]广西中医药大学,南宁市530001

出　　处：《内科》2015年第6期765-770,共6页Internal Medicine

基　　金：国家自然科学基金项目(81160455);广西高校中医药防治肥胖症重点实验室培育基地

摘　　要：目的探讨针刺对肥胖大鼠慢性炎症状态的调节作用。方法 60只雄性SD大鼠普通饲料适应性喂养3 d后,随机分为两组,分别喂食普通饲料(空白对照组10只)和高脂饲料(50只)。50只高脂饲料组大鼠喂养7周后,体重超过普通饲料组大鼠平均体重20%者37只,从其中随机选择30只,分为肥胖模型对照组(10只)、非特定穴位针刺组(10只)、特定穴位针刺组(10只),干预4周后,麻醉大鼠、心脏采血,采用酶联免疫法检测血清游离脂肪酸、血清三酰甘油、总胆固醇,以及血清IL-6、TNF-α。结果 (1)高脂饮食诱导的大鼠体重、附睾脂肪、肾周脂肪质量明显高于空白对照组(P<0.01),与肥胖模型对照组大鼠比较,非特定穴位针刺组及特定穴位针刺组大鼠体重及附睾脂肪、肾周脂肪质量均明显下降(P<0.05或P<0.01);(2)高脂饮食诱导的大鼠血清游离脂肪酸、血清三酰甘油、总胆固醇,以及血清IL-6,TNF-α均明显高于空白对照组(P<0.01),而针刺干预可明显降低高脂饮食诱导肥胖大鼠血清游离脂肪酸、血清三酰甘油、总胆固醇,以及血清IL-6,TNF-α水平(P<0.05或P<0.01);(3)与非特定穴位针刺组比较,特定穴位针刺组大鼠体重、肾周脂肪、附睾脂肪数,以及血清IL-6明显下降(P<0.05)。结论 (1)针刺治疗具有显著的减肥功效;针刺减肥的作用机制可能与抑制慢性炎症状态相关;(2)中医药理论指导下的特定穴位针刺治疗比非经络上穴位治疗效果更好。Objective To explore the regulatory effect of acupuncture on chronic inflammation in rat model of nutritional obesity. Methods Sixty Sprague-Dawley rats of maleness were selected and fed with normal diet for 3 days, then were randomly divided into the blank control group （fed with normal diet, 10 rats ） and high fat diet group （fed with high fat diet, 50 rats） , after 7 weeks of high fat diet, 37 of the 50 rat in high fat diet group had successfully get the obesity diagnosis standard, 30 of them were selected and randomly divided into three groups （ obesity control group, non-specific aeupoint acupuncture group and specific aeupoint acupuncture group） with 10 rats in each group. After intervention for 4 weeks, rats were anesthetized and the level of serum FFA, triglyceride, total cholesterol, serum IL-6 and TNF-α were tested by euzymelinked immunosorbent assay. Results （1） The level of rat body weight, epididymis and perirenal fat in the high fat diet group were significantly higher than those in blank control group （P 〈 0.01 ） ; and these indexes in specific acupoint acupuncture group and non-specific aeupoint acupuncture group were significantly lower than those in obesity control group（P 〈0.05 or P 〈 0.01 ）. （2） The level of serum FFA, triglyceride, total cholesterol, serum IL-6 and TNF-α in the high fat diet group were significantly higher than those in blank control group （ P 〈 0. 01 ） ; and these indexes in specific acupoint acupuncture group and non-specific aeupoint acupuncture group were significantly lower than those in obesity control group （P 〈 0.05 or P 〈 0. 01 ）. （ 3 ） The level of rat body weight, epididymis, perirenal fat and serum IL-6 in the specific aeupoint acupuncture group were significantly lower than those in non-specific acupoint acupuncture group（P 〈 0.05 ）. Conclusions （1） Acupuncture treatment has remarkable effect on reducing weight and its mechanism is related to regulating the chronic inflammation; （2） the therapeut

关 键 词：慢性炎症 针刺 特定穴位 

分 类 号：R245[医药卫生—针灸推拿学]