合并HIV感染肺结核患者Mtb的MLVA与PFGE法基因分型对比研究  

A comparative study of PFGE and MLVA to genotype Mycobacterium tuberculosis infecting patients with HIV in Dali District

作　　者：王旭东[1] 聂恒[1] 吴利先[1] 

机构地区：[1]大理学院基础医学院微生物学与免疫学教研室,云南大理671000

出　　处：《中国病原生物学杂志》2015年第12期1082-1085,1096,共5页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.81260456)

摘　　要：目的对比PFGE和MLVA两种不同分型方法用于大理地区合并HIV感染肺结核患者Mtb临床分离株基因分型的优劣。方法选取大理地区27株合并HIV感染肺结核患者Mtb临床分离株,采用PFGE和MLVA两种方法进行基因分型,应用BioNumerics(6.6)软件进行聚类分析。结果 PFGE法将27株Mtb分为5个基因群,其中主要优势基因群为A群(15株)包括H37Rv,D=0.698;MLVA法将27株Mtb分为4个基因群,其中主要优势基因群为Ⅰ群(8株,包括H37Rv减毒株)和Ⅱ群(9株)。D=0.999;对比分析显示MLVA法分辨力高于PFGE法。结论 MLVA法与PFGE法相比更适合于大理地区合并HIV感染肺结核患者Mtb的基因分型。Objective To compare PFGE and MLVA to genotype Mycobacterium tuberculosis infecting patients with HIV in Dali District. Methods Clinical isolates of 27 strains of M.tuberculosisinfecting patients with HIV in Dali district were studied.PFGE and MLVA were used to genotype M.tuberculosis and results were analyzed with BioNumerics（6.6）. Results All 27 strains of M.tuberculosis fell into 5different genotypes according to PFGE.The predominant genotype was genotype A（14strains）,which included the H37 Rv strain（D=0.698）.Strains of M.tuberculosis fell into4 different genotypes according to MLVA.The predominant genotypes were genotypes I（8strains,including H37Rv）and II（9strains）（D=0.999）.Comparative analysis indicated that MLVA had a higher resolution than PFGE. Conclusion MLVA is better than PFGE at genotyping M.tuberculosis infecting patients with HIV in Dali District..

关 键 词：结核分枝杆菌 基因分型 MLVA PFGE 双重感染 

分 类 号：R521[医药卫生—内科学] R512.91[医药卫生—临床医学]