奶牛子宫内膜炎病原菌分离与药敏试验  

Identification and Drug Sensitivity Test of the Pathogenic Microbe of Dairy Cattle Endometritis

作　　者：郭素风 杨忠 杨继业 张元庆[3] 程景[3] 王曦[3] 张喜忠[3] 王栋才[3] 孙锐锋[3] 靳光[3] 杨效民[3] 

机构地区：[1]和顺县畜牧局,山西和顺032700 [2]山西省畜牧遗传育种中心,山西太原030027 [3]山西省农业科学院畜牧兽医研究所,山西太原030032

出　　处：《中国牛业科学》2015年第6期24-27,共4页China Cattle Science

基　　金："十二五"国家高技术研究发展计划(863计划)子课题(2011AA100307-05);农业部公益性行业专项(201503134);动物营养学国家重点实验室开放课题(2004DA125184F1028);山西省科技攻关项目(20130311024-2;20120311021-1);山西省农业科学院育种工程项目(11yzgc017)

摘　　要：[目的]为了准确高效防治牛子宫内膜炎。[方法]对30头病例的病原菌分离与药敏试验,结果从30头患畜子宫分泌物中分离鉴定出3种主要致病菌,其中疑似葡萄球菌14株,阳性率为46.7%(14/30);疑似链球菌11株,阳性率为36.7%(11/30);疑似大肠杆菌5株,阳性率为16.7%(5/30)。[结果]表明:恩诺沙星、卡拉霉素、诺氟沙星的抑菌效果明显,可视为目标场治疗子宫内膜炎的首选药物。而链霉素、青霉素的抑菌效果相对较差,可能与其长期使用这些药物而产生耐药性有关。[结论]用药时需合理配伍,不随意加大剂量,同时几种药物交替使用,以免产生耐药菌株,影响治疗效果。[Objective] Cattle endometritis is a common and frequently-occurring disease, which majorly affects the conception rate in dairy cattle farm. The objective of this study is to prevent and cure cattle en-dometritis accurately and efficiently. [Method] Pathogens were separated from 30 dairy cattle that suffered from endometritis and were analyzed by medicine sensitive test. The results showed that the uterine scoretion derived pathogens included three major kinds of baeterias. 14 strains of suspected Staphylococcus au reus （positive rate is 46.7%）, 11 strains of suspected Streptococcus （positive rate is 36.7%）, 5 strains suspected Escherichia coli （positive rate is 16. 7%）. [Result] Based on the medicine sensitive test, showed that enrofloxacin, kalamycin, norfloxacin may observably inhibit the growth and proliferation of it of pathogens and may be the primary candidate for endometritis therapy in dairy cattle farm. However, the pathogens were insensitive to streptomycin and penicillin. It suggested that drug-- resistant generated and may due to long--term use of these antibiotics. [Conclusion] As a conclusion, to avoid drug--resistant and maximize the therapeutic efficacy, compatibility of medicines should be applied in a reasonable ratio and the dosage should be controlled and should not be increased casually. Meanwhile, different kinds of medicine should be used alternately.

关 键 词：奶牛子宫内膜炎 发病率 病原菌 药敏试验 治疗药物选择 

分 类 号：S858.237.2[农业科学—临床兽医学]