亚砷酸钠对PC12细胞色素上皮衍生因子及凋亡相关因子mRNA表达的影响  

The effects of sodium arsenite on mRNA expression level of pigment epithelimn-derived factor and apoptosis-related factors in PC12 cells

作　　者：崔晓慧[1] 张微[1] 李媛媛[1] 孙丽艳[2] 高彦辉[1] 姜丽欣[1] 曲丽莎[1] 刘晓娜[1] 张锦慧[1] 吕春鹏[1] 孙殿军[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心地氟病防治研究所,哈尔滨150081 [2]哈尔滨医科大学大骨节病防治研究所,哈尔滨150081

出　　处：《中华地方病学杂志》2016年第1期10-13,共4页Chinese Journal of Endemiology

基　　金：高等学校博士学科点专项科研基金（20112307130003）

摘　　要：目的观察不同剂量亚砷酸钠（NaAsO2）对PCI2细胞（具有神经元特性的大鼠嗜铬瘤细胞）色素上皮衍生因子（PEDF）和凋亡相关因子mRNA表达的影响。方法选择PC12细胞作为研究对象，给予不同剂量的NaAsO2[0（对照组）、2、5、10μmol／L]染毒24h，使用Real-time荧光定量PCR检测不同剂量NaAsO2染毒后PC12细胞的PEDF及其凋亡相关因子Bax和Bcl-2mRNA的表达水平。结果PEDFmRNA表达组间比较差异有统计学意义（F=102．28，P〈0．05），其中2、5、10μmol／LNaAsO2染毒组的PEDFmRNA表达（0．70±0．07、0．33±0．04、0．23±0．10）均低于对照组（1．15±0．11，P〈0．05）。BaxmRNA表达组间比较差异无统计学意义（0、2、5、10μmoVL组分另0为0．95±0．12、0．80±0．11、0．88±0．11、1．01±0．11，F=2．01，P〉0．05）；Bcl-2mRNA表达组间比较差异有统计学意义（F=19．87，P〈0．05），其中2、5、10μmol／LNaAs02染毒组Bcl-2mRNA表达（0．65±0．03、0．49±0．04、0．57±0．09）均低于对照组（0．95±0．11，P均〈0．05）；Bax／Bcl-2mRNA表达比值组间比较差异有统计学意义（F=8．35，P〈0．05），其中5、10μmol／LNaAsO2染毒组的Bax／Bcl-2mRNA表达比值（1.80±0．72、1．82±0．36）高于对照组（1．02±0．24，P均〈0．05）。结论NaAsO2可能通过降低PC12细胞PEDFmRNA的表达来增加凋亡相关因子Bax／Bcl-2mRNA表达．继而引起细胞凋亡。Objective To observe the effects of different levels of sodium arsenite （NaAsO2） on mRNA expression of pigment epithelium-derived factor （PEDF） and apoptosis-related factors in PC12 cells （rat neuron properties pheochromocytoma）. Methods PC12 cells were treated with different levels of NaAsO2 [0 （control group）, 2, 5, 10 μmol/L] for 24 hours. The mRNA expression of PEDF and apoptosis-related factors （Bax, Bcl-2） were detected by real-time quantitative PCR. Results There were significant differences in the mRNA expressions of PEDF between the 4 groups （F = 102.28, P 〈 0.05）, the mRNA expressions of PEDF in the group of 2, 5, 10 μmol/L （0.70 ± 0.07, 0.33 ± 0.04, 0.23 ± 0.10） was lower than that of control group （1.15 ±0.11, P 〈 0.05）; there were no significant differences in the mRNA expressions of Bax between the 4 groups （0, 2, 5, 10 μmol/L groups： 0.95 ± 0.12, 0.80 ± 0.11, 0.88 ± 0.11, 1.01 ± 0.11, F = 2.01, P 〉 0.05）; there were significant differences in the mRNA expressions of Bcl-2 between the 4 groups （F = 19.87, P 〈 0.05）, the mRNA expressions of Bcl-2 in the group of 2, 5, 10 μmol/L （0.65 ± 0.03, 0.49 ± 0.04, 0.57 ± 0.09） were lower than that of control group （0.95 ± 0.11, all P 〈0.05）; there were significant differences in the mRNA expressions of Bax/Bel-2 between the 4 groups （F = 8.352, P 〈 0.05）, the mRNA expressions of in the group of 5, 10 μmol/L （1.80 ± 0.72, 1.82 ± 0.36） were higher than that of control group （1.02± 0.24, all P 〈 0.05）. Conclusion NaAsO2 may increase the expression of apoptosis-related factorsBax/ Bcl-2 mRNA by decreasing the expression of PEDF mRNA in PC12 cells, leading to apoptosis in PC12 cells.

关 键 词：亚砷酸钠 PC12细胞 色素上皮衍生因子 凋亡相关因子 

分 类 号：R114[医药卫生—卫生毒理学]