砖茶氟暴露对人群血清骨形态发生蛋白2表达水平的影响  

Influence of brick-tea fluoride exposure on levels of bone morphogenetic protein 2 expression in human serum

作　　者：范智鹏[1] 孙静[2] 刘洋[1] 李丙云[1] 郦芒[1] 刘晓娜[1] 孙殿军[1] 高彦辉[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心国家卫生计生委病因流行病学重点实验室、黑龙江省普通高校病因流行病学重点实验室地氟病防治研究所,哈尔滨150081 [2]宁夏回族自治区疾病预防控制中心地方病科,银川750004

出　　处：《中华地方病学杂志》2016年第1期18-22,共5页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81172605）

摘　　要：目的探讨长期饮用砖茶导致的高氟暴露对人群血清骨形态发生蛋白2（BMP-2）表达的影响。方法在青海果洛州藏族、内蒙古呼伦贝尔市蒙古族及新疆阿勒泰哈萨克族居住地区的典型饮茶型氟中毒病区，选取典型成人氟骨症患者73例，按照年龄（相差不超过3岁）、性别和民族匹配无氟骨症对照73例。对调查对象进行问卷调查，对其前臂、腰椎及骨盆关节拍摄X线片，并采集调查对象饮用的茶水（或酥油茶）样、即时尿样和全血样本。茶水氟和尿氟含量检测采用氟离子选择电极法（WS／T89—2006），血清BMP-2含量检测采用酶联免疫吸附试验（ELISA）。依据日均茶水氟摄入量、尿氟含量四分位数及不同程度氟骨症病情将被调查对象分别分为4组,进行进一步分析。结果共调查146人，人群血清BMP-2含量在不同年龄组及性别间比较差异均无统计学意义（χ^=2．95、U=2148．50，P均〉0．05）；人群血清BMP-2含量在不同民族间比较差异有统计学意义（χ^=36．28，P〈0．01），其中藏族人群血清BMP-2含量高于哈萨克族人群（中位数：109．91比73．96ng／L，P〈0．01），且藏族人群的尿氟含量显著低于哈萨克族人群（中位数：2．23比3．42mg／L，U=672．00，P〈0．01），但两组人群的日均茶水氟摄入量比较差异无统计学意义（中位数：7．05比7．69ng／L，U=674．00，P〉0．05）。不同程度茶水氟暴露组和不同含量尿氟组人群血清BMP-2含量比较差异均无统计学意义（χ^2=7．34、1．94，P均〉0．05）。茶水氟暴露和尿氟与人群血清BMP-2表达统计学分析无相关性（r=-0．037、-0．015，P均〉0．05）。病例组与对照组人群血清BMP-2含量比较差异无统计学意义（中位数：85．76比98．18ng／L，Z=-1．78，P〉0．05）。不同程度氟骨症（正常、轻度、中度、重度组）组间人群血清BMP-2含量比较差异无统计�Objective To find out whether high fluoride exposure could affect the bone morphogenetic protein 2 （BMP-2） expression in human serum. Methods In the typical brick tea-type fluorosis areas of Guoluo Autonomous Prefecture of Qinghai Province, Hulunbeir City of Inner Mongolia Autonomous Region and Ahay Prefecture of Xinjiang Autonomous Region, 73 patients with skeletal fluorosis in the typical brick-tea type fluorosis districts were selected. Accordingly, 73 age （difference not more than 3 years old）, sex and nationality matched healthy controls were selected. The volunteers were investigated by questionnaire, and brick-tea water （or buttered tea）, urine and peripheral blood samples were collected. Forearm, lumbar and pelvic joints were taken X-rays. The fluoride concentrations in brick-tea water （or buttered tea） and urine were determined by fluoride ion selective electrode method （WS/T 89-2006）. The concentration of BMP-2 in human serum was quantified by enzyme linked immunosorbent assay （ELISA）. The respondents were divided into 4 groups according to average daily fluoride intake, urine fluoride quartiles and different conditions of skeletal fluorosis, and further study was conducted. Results Totally 146 subjects were investigated. The concentrations of BMP-2 in human serum among the groups with different ages were not significantly different （χ^2 = 2.95, P 〉 0.05）. The concentrations of BMP-2 in human serum between female and male population were not significantly different （U = 2148.50, P 〉 0.05 ）. However, compared with the concentration of BMP-2 in Kazakans＇ serum （median： 73.96 ng/L）, the concentration of BMP-2 in Tibetans＇ serum was significantly higher （median： 109.91 ng/L, χ^2= 36.28, P 〈 0.01）. The average daily fluoride intake of brick-tea water was not significantly different between Tibetans and Kazakans （median： 7.69 vs. 7.05 ng/L, U = 674.00, P 〉 0.05）. However, the urine fluoride concentration in Kazakans was significantly higher compa

关 键 词：氟 骨骼 骨形态发生蛋白2 血清 

分 类 号：R599.1[医药卫生—内科学]