桂林毛村不同植被类型土壤微生物数量与碳酸酐酶活性比较  

Comparison on Microbial Quantity and Carbonic Anhydrase Activity in Soil under Different Vegetation in Maocun,Guilin

作　　者：黄芬[1,2,3] 黄艳梅[1,2,3] 杨利超[1,2,3] 白冰[1,2,3] 曹建华[1,2,3] 

机构地区：[1]中国地质科学院岩溶地质研究所,广西桂林541004 [2]国土资源部、广西岩溶动力学重点实验室,广西桂林541004 [3]联合国教科文组织国际岩溶研究中心,广西桂林541004

出　　处：《地质论评》2016年第1期94-102,共9页Geological Review

基　　金：国家自然科学基金项目(编号:41302289);广西自然科学基金项目(编号:2014GXNSFBA118225);中国地质调查项目(编号:12120113005300);国土资源部公益性行业科研专项基金项目(编号:201211086-05);广西科学研究与技术开发计划项目(编号:桂科合14125008-2-1)资助的成果~~

摘　　要：以非岩溶区林地为对比，分析了桂林毛村岩溶区4种不同植被类型土壤微生物数量及碳酸酐酶（CA）活性的季度动态变化规律，发现以下主要结果：①随着植被的正向演替，岩溶区弃耕地、草地、灌丛及林地微生物数量及CA活性逐渐升高，微生物总数从64．07×10^4cfu／g上升到178．23×10^4cfu／g，CA活性从0．77U／g上升到1．82U／g，岩溶区林地大于非岩溶区林地。②在岩溶区不同植被类型，微生物组成均表现为细菌最多（平均值95．14％），放线菌次之（平均值2．79％），真菌数量最少（平均值1．75％）。而在非岩溶区表现为细菌最多（平均值90．95％），真菌次之（平均值5．32％），放线菌最少（平均值3．73％）。③微生物数量季节动态整体表现为春季至夏季逐渐上升，至秋季达到最高，冬季下降，微生物总数的增长依赖于细菌的倍数增长，真菌和放线菌影响较小。CA活性整体表现为夏季和冬季低于春季和秋季，秋季达到最大值。④CA活性与细菌及微生物总数呈极显著的正相关，表明土壤CA主要来源于细菌的分泌。Objectives： New findings have shown that the limestone tablet dissolutional rate in soil increases along with the positive succession of vegetation. The driving force of the tablet dissolution is commonly CO2 and H20, and their interaction. Soil micorobes strongly impact the CO2 concentration and cycle, and soil carbonic anhydrase （CA） impacts the interaction of the CO2 ＋ H20, however very few studies have been conducted to investigate the changes in soil microbe and CA activity along with the positive succession of vegetation. Methods： In this study, soil samples （0- 20cm depth） in different seasons （i. e., spring, summer, autumn and winter） from four vegetation types including derelict land, grass land, brushwood, and woodland, were sampled in Maocun karst catchment, Guilin; soils under woodland from adjacent non -karst region were also collected as control. Soil microbial quantity and CA activity were measured using dilution--plate method and pH probe, respectively. Results： Total microbial quantity and CA activity were significantly affected by vegetation types, sampling times and regions. Total microbial quantity in soils ranged from 64.07 x 104 cfu/g in derelict land to 178.23 x 104 cfu/g in woodland and CA activity ranged from 0.77 U/g in derelict land to 1.82 U/g in woodland in karst region. In contrast to karst region, both microbial quantity and CA activity in soils under woodland were significantly lower in non-karst region. In karst region, the quantity ratios of bacteria, aetinomyces and fungus were 95. 14% , 2. 79% , 1.75% , respectively, while in the non-karst area, the quantity ratios of bacteria, fungus and actinomyces were 90.95% , 5.32% and 3.73%. The seasonal variation of the microbial quantity was in increasing trend from spring to summer, and decreased in winter after reaching the maximum in autumn. The total soil microbial quantity was dominately composed of the bacteria, while the fungus and actinomyces gave little impact. The trends of CA activity were higher in spri

关 键 词：植被类型 微生物数量 碳酸酐酶 岩溶流域 桂林毛村 

分 类 号：S154[农业科学—土壤学]